ArticlePloS one2011
Multidimensional single cell based STAT phosphorylation profiling identifies a novel biosignature for evaluation of systemic lupus erythematosus activity.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 46 citations in OpenAlex.
- Single-cell Unperturbed Activation Profiling by mass cytometry capturesFrontiers in immunology · 2026Article
- Impaired activation of STAT5 upon IL-2 stimulation in Tregs and elevated sIL-2R in Sjögren's syndrome.Arthritis research & therapy · 2022Article
- SCARF1-Induced Efferocytosis Plays an Immunomodulatory Role in Humans, and Autoantibodies Targeting SCARF1 Are Produced in Patients with Systemic Lupus Erythematosus.Journal of immunology (Baltimore, Md. : 1950) · 2022Article
- The effect of prolactin on immune cell subsets involved in SLE pathogenesis.Frontiers in immunology · 2022Review
- Cytokines and Transgenic Matrix in Autoimmune Diseases: Similarities and Differences.Biomedicines · 2020Review
- Upregulated expression of STAT3/IL-17 in patients with systemic lupus erythematosus.Clinical rheumatology · 2019Article
- Aberrant STAT phosphorylation signaling in peripheral blood mononuclear cells from multiple sclerosis patients.Journal of neuroinflammation · 2018Article
- Prolactin has a pathogenic role in systemic lupus erythematosus.Immunologic research · 2017Article
- Activated Platelets Induce Endothelial Cell Activation via an Interleukin-1β Pathway in Systemic Lupus Erythematosus.Arteriosclerosis, thrombosis, and vascular biology · 2017Article
- The Role of STAT Signaling Pathways in the Pathogenesis of Systemic Lupus Erythematosus.Clinical reviews in allergy & immunology · 2017Review
- Autoantibody-Positive Healthy Individuals Display Unique Immune Profiles That May Regulate Autoimmunity.Arthritis & rheumatology (Hoboken, N.J.) · 2016Article
- STAT3 Signaling in B Cells Is Critical for Germinal Center Maintenance and Contributes to the Pathogenesis of Murine Models of Lupus.Journal of immunology (Baltimore, Md. : 1950) · 2016Article
- Signal transducer and activator of transcription 5 is implicated in disease activity in adult and juvenile onset systemic lupus erythematosus.Clinical rheumatology · 2016Article
- Development of Th17-Associated Interstitial Kidney Inflammation in Lupus-Prone Mice Lacking the Gene Encoding STAT-1.Arthritis & rheumatology (Hoboken, N.J.) · 2016Article
- STAT-5 is activated constitutively in T cells, B cells and monocytes from patients with primary Sjögren's syndrome.Clinical and experimental immunology · 2015Article
- Overexpression of microRNA-155 increases IL-21 mediated STAT3 signaling and IL-21 production in systemic lupus erythematosus.Arthritis research & therapy · 2015Article
- NKG2D ligand overexpression in lupus nephritis correlates with increased NK cell activity and differentiation in kidneys but not in the periphery.Journal of leukocyte biology · 2015Article
- Impaired T cell responsiveness to interleukin-6 in hematological patients with invasive aspergillosis.PloS one · 2015Article
- Stat3 promotes IL-10 expression in lupus T cells through trans-activation and chromatin remodeling.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Systemic lupus erythematosus diagnostics in the 'omics' era.International journal of clinical rheumatology · 2013Article
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
introductionDysregulated cytokine action on immune cells plays an important role in the initiation and progress of systemic lupus erythematosus (SLE), a complex autoimmune disease. Comprehensively quantifying basal STATs phosphorylation and their signaling response to cytokines should help us to better understand the etiology of SLE.
methodsPhospho-specific flow cytometry was used to measure the basal STAT signaling activation in three immune cell types of peripheral-blood mononuclear cells from 20 lupus patients, 9 rheumatoid arthritis (RA) patients and 13 healthy donors (HDs). A panel of 27 cytokines, including inflammatory cytokines, was measured with Bio-Plex™ Human Cytokine Assays. Serum Prolactin levels were measured with an immunoradiometric assay. STAT signaling responses to inflammatory cytokines (interferon α [IFNα], IFNγ, interleukin 2 [IL2], IL6, and IL10) were also monitored.
resultsWe observed the basal activation of STAT3 in SLE T cells and monocytes, and the basal activation of STAT5 in SLE T cells and B cells. The SLE samples clustered into two main groups, which were associated with the SLE Disease Activity Index 2000, their erythrocyte sedimentation rate, and their hydroxychloroquine use. The phosphorylation of STAT5 in B cells was associated with cytokines IL2, granulocyte colony-stimulating factor (G-CSF), and IFNγ, whereas serum prolactin affected STAT5 activation in T cells. The responses of STAT1, STAT3, and STAT5 to IFNα were greatly reduced in SLE T cells, B cells, and monocytes, except for the STAT1 response to IFNα in monocytes. The response of STAT3 to IL6 was reduced in SLE T cells.
conclusionsThe basal activation of STATs signaling and reduced response to cytokines may be helpful us to identify the activity and severity of SLE.
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