ArticleChemical engineering science2010
Efficiency of the Polymerase Chain Reaction.
Article in Chemical engineering science, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
17 citing papers in PubMed.
- DYNAMIC: A Novel Software Implementation of a Kinetic Model of TaqMan PCR.Analytical chemistry · 2026Article
- Feline enteropathogens and molecular diagnostics: benefits, limitations and clinical applications.Journal of feline medicine and surgery · 2025Review
- A Real-Time PCR Assay for Detecting Codling MothInternational journal of molecular sciences · 2025Article
- PCR in Forensic Science: A Critical Review.Genes · 2024Review
- Error-suppression mechanism of PCR by blocker strands.Biophysical journal · 2023Article
- Characterisation of Primary Human Hippocampal Astrocyte Cell Culture Following Exposure to Hypoxia.The Malaysian journal of medical sciences : MJMS · 2023Article
- Assay Optimization Can Equalize the Sensitivity of Real-Time PCR with ddPCR for Detection ofInsects · 2021Article
- Article
- DNA degradation in human teeth exposed to thermal stress.Scientific reports · 2021Article
- Integrated magneto-electrophoresis microfluidic chip purification on library preparation device for preimplantation genetic testing for aneuploidy detection.RSC advances · 2021Article
- Sampling Variation of RAD-Seq Data from Diploid and Tetraploid Potato (Plants (Basel, Switzerland) · 2021Article
- Variability in CharacterizingMicroorganisms · 2018Article
- Detection of Typhoidal and Paratyphoidal Salmonella in Blood by Real-time Polymerase Chain Reaction.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2015Article
- Multi-template polymerase chain reaction.Biomolecular detection and quantification · 2014Review
- Analysis of qPCR reference gene stability determination methods and a practical approach for efficiency calculation on a turbot (Scophthalmus maximus) gonad dataset.BMC genomics · 2014Article
- Article
- Experimental Validation of a Fundamental Model for PCR Efficiency.Chemical engineering science · 2011Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
Abstract
The polymerase chain reaction (PCR) has found wide application in biochemistry and molecular biology such as gene expression studies, mutation detection, forensic analysis and pathogen detection. Increasingly quantitative real time PCR is used to assess copy numbers from overall yield. In this study the yield is analyzed as a function of several processes: (1) thermal damage of the template and polymerase occurs during the denaturing step, (2) competition exists between primers and templates to either anneal or form dsDNA, (3) polymerase binding to annealed products (primer/ssDNA) to form ternary complexes and (4) extension of ternary complexes. Explicit expressions are provided for the efficiency of each process, therefore reaction conditions can be directly linked to the overall yield. Examples are provided where different processes play the yield-limiting role. The analysis will give researchers a unique understanding of the factors that control the reaction and will aid in the interpretation of experimental results.
Identifiers
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.