ArticleNature protocols2011
Selection of proteins with desired properties from natural proteome libraries using mRNA display.
Article in Nature protocols, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
15 citing papers in PubMed.
- Cell-Free Gene Expression: Methods and Applications.Chemical reviews · 2025Review
- Revealing protein-protein interactions at the transcriptome scale by sequencing.Molecular cell · 2021Article
- Directing evolution of novel ligands by mRNA display.Chemical Society reviews · 2021Review
- Review
- Cancer Immunotherapy with T Cells Carrying Bispecific Receptors That Mimic Antibodies.Cancer immunology research · 2019Article
- Nucleic Acid-Barcoding Technologies: Converting DNA Sequencing into a Broad-Spectrum Molecular Counter.Angewandte Chemie (International ed. in English) · 2019Review
- LOVTRAP: an optogenetic system for photoinduced protein dissociation.Nature methods · 2016Article
- In Vitro Selection of Cancer Cell-Specific Molecular Recognition Elements from Amino Acid Libraries.Journal of immunology research · 2015Review
- Peptide aptamers: development and applications.Current topics in medicinal chemistry · 2015Review
- Liposome display for in vitro selection and evolution of membrane proteins.Nature protocols · 2014Article
- Creation of recombinant antigen-binding molecules derived from hybridomas secreting specific antibodies.Nature protocols · 2013Article
- In vitro selection of proteins with desired characteristics using mRNA-display.Methods (San Diego, Calif.) · 2013Article
- Streamlined protocol for mRNA display.ACS combinatorial science · 2013Article
- Development of Next-Generation Peptide Binders Using In vitro Display Technologies and Their Potential Applications.Frontiers in immunology · 2013Article
- In vitro evolution of enzymes.Methods in molecular biology (Clifton, N.J.) · 2013Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
Abstract
mRNA display is a powerful yet challenging in vitro selection technique that can be used to identify proteins with desired properties from both natural proteome and combinatorial polypeptide libraries. The physical conjugation between a protein and its own RNA presents unique challenges in manipulating the displayed proteins at a low nanomolar scale in an RNase-free environment. The following protocol outlines the generation of cDNA libraries derived from natural organisms as well as the steps required for generation of mRNA-protein fusion molecules, in vitro functional selection and regeneration of the selected cDNA library. The selection procedures for the identification of protease substrates and Ca(2+)-dependent calmodulin-binding proteins from natural cDNA libraries are presented as examples. The method can be generally applied to the identification of protein sequences with desired properties from various natural proteome libraries. One round of mRNA display-based selection can be accomplished in ~7 d.
Indexed as
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21799486What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.