ArticleSkeletal muscle2011
Sequential association of myogenic regulatory factors and E proteins at muscle-specific genes.
Article in Skeletal muscle, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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Who cites it
45 citing papers in PubMed, 85 citations in OpenAlex.
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- Muscle cell-type diversification is driven by bHLH transcription factor expansion and extensive effector gene duplications.Nature communications · 2023Article
- Tcf12 is required to sustain myogenic genes synergism with MyoD by remodelling the chromatin landscape.Communications biology · 2022Article
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- SIX1 reprograms myogenic transcription factors to maintain the rhabdomyosarcoma undifferentiated state.Cell reports · 2022Article
- Changes in Elastic Moduli of Fibrin Hydrogels Within the Myogenic Range Alter Behavior of Murine C2C12 and Human C25 Myoblasts Differently.Frontiers in bioengineering and biotechnology · 2022Article
- Myostatin and its Regulation: A Comprehensive Review of Myostatin Inhibiting Strategies.Frontiers in physiology · 2022Review
- Myogenin controls via AKAP6 non-centrosomal microtubule-organizing center formation at the nuclear envelope.eLife · 2021Article
- Deletion of phosphatidylserine flippase β-subunitZoological research · 2021Article
- Myonuclear transcriptional dynamics in response to exercise following satellite cell depletion.iScience · 2021Article
- Myogenin is required for assembly of the transcription machinery on muscle genes during skeletal muscle differentiation.PloS one · 2021Article
- The PRC2 complex directly regulates the cell cycle and controls proliferation in skeletal muscle.Cell cycle (Georgetown, Tex.) · 2020Article
- IFN-γ and CIITA modulate IL-6 expression in skeletal muscle.Cytokine: X · 2020Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
backgroundGene expression in skeletal muscle is controlled by a family of basic helix-loop-helix transcription factors known as the myogenic regulatory factors (MRFs). The MRFs work in conjunction with E proteins to regulate gene expression during myogenesis. However, the precise mechanism by which the MRFs activate gene expression is unclear. In this work, we sought to define the binding profiles of MRFs and E proteins on muscle-specific genes throughout a time course of differentiation.
resultsWe performed chromatin immunoprecipitation (ChIP) assays for myogenin, MyoD, Myf5 and E proteins over a time course of C2C12 differentiation, resulting in several surprising findings. The pattern of recruitment is specific to each promoter tested. The recruitment of E proteins often coincides with the arrival of the MRFs, but the binding profile does not entirely overlap with the MRF binding profiles. We found that E12/E47 is bound to certain promoters during proliferation, but every gene tested is preferentially bound by HEB during differentiation. We also show that MyoD, myogenin and Myf5 have transient roles on each of these promoters during muscle differentiation. We also found that RNA polymerase II occupancy correlates with the transcription profile of these promoters. ChIP sequencing assays confirmed that MyoD, myogenin and Myf5 co-occupy promoters.
conclusionsOur data reveal the sequential association of MyoD, myogenin, Myf5 and HEB on muscle-specific promoters. These data suggest that each of the MRFs, including Myf5, contribute to gene expression at each of the geness analyzed here.. The dynamic binding profiles observed suggest that MRFs and E proteins are recruited independently to promoters.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.