ArticleBMC research notes2011
SYTO dyes and EvaGreen outperform SYBR Green in real-time PCR.
Article in BMC research notes, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
50 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Pooled it
- Mechanism and application of Taq DNA polymerase in TaqMan qPCR.Frontiers in bioengineering and biotechnology · 2026Article
- Dual-Emission FRET-PCR Outperforms SYBR Green and EvaGreen for Accurate Discrimination of Primary Canine Dermatophytes:Journal of fungi (Basel, Switzerland) · 2025Article
- Performance and functional assessment of the Kimera P-IV point-of-care plasmonic qPCR prototype for ultra rapid pathogen detection ofEpidemiology and infection · 2025Article
- Development of a High-Resolution Melting Method for the Detection of Clarithromycin-ResistantAntibiotics (Basel, Switzerland) · 2024Article
- Chloroplast mini-barcodes combined with high resolution melting analysis to identify herbal medicine Difengpi (Heliyon · 2024Article
- Review
- Evaluation of A Simple DNA Extraction Method and Its Combination with Loop-Mediated Isothermal Amplification Assays for RapidTropical medicine and infectious disease · 2023Article
- Binding, brightness, or noise? Extracting temperature-dependent properties of dye bound to DNA.Biophysical journal · 2023Article
- Recent Advances in Digital Biosensing Technology.Biosensors · 2022Review
- Development of Droplet Digital PCR Assay for Detection of Seed-BorneMicroorganisms · 2022Article
- Toward a next-generation diagnostic tool: A review on emerging isothermal nucleic acid amplification techniques for the detection of SARS-CoV-2 and other infectious viruses.Analytica chimica acta · 2022Review
- Two Leishmania species separation targeting the ITS-rDNA and Cyt b genes by developing and evaluating HRM- qPCR.Revista da Sociedade Brasileira de Medicina Tropical · 2022Article
- Optimization and development of high-resolution melting curve analysis (HRMA) assay for detection of New Delhi metallo-β-lactamase (NDM) producingAIMS microbiology · 2022Article
- Identification of extremely GC-rich micro RNAs for RT-qPCR data normalization in human plasma.Frontiers in genetics · 2022Article
- Homebrew reagents for low-cost RT-LAMP.Journal of biomolecular techniques : JBT · 2021Article
- Homebrew reagents for low cost RT-LAMP.medRxiv : the preprint server for health sciences · 2021Article
- A cost-effective and efficient approach for generating and assembling reagents for conducting real-time PCR.Journal of biosciences · 2021Article
- A quantitative loop-mediated isothermal amplification assay for detecting a novel goose astrovirus.Poultry science · 2020Article
- High-throughput methods for measuring DNA thermodynamics.Nucleic acids research · 2020Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
1 author.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundReal-time PCR can be carried out using either probes or DNA dyes. SYBR Green has been used the most, but it suffers from several drawbacks. Numerous other DNA dyes are commercially available, but with limited structural information. Dye behavior in real time PCR is difficult to predict, so empirical data are needed. In the work described here, a panel of 23 different DNA dyes--including green, orange, and red SYTO dyes, EvaGreen, and SYBR Green--were evaluated with respect to their performance in real time PCR.
findingsData were analyzed for reaction inhibition, effects on amplicon melting temperature, fluorescent signal strength, and reaction efficiency. This is the first report of reaction efficiency using alternatives to SYBR Green. Results indicated substantial variation in performance even within the SYTO dye family. EvaGreen and the SYTO dyes 13, 16, 80, and 82 performed better than SYBR Green in general, and high reaction efficiencies can be achieved using these dyes.
conclusionsEmpirical data were generated for 23 DNA dyes. This paper confirms and extends previous findings that among commercially available DNA dyes, EvaGreen and certain SYTO dyes are the most desirable alternatives to the commonly used SYBR Green in real-time PCR.
Identifiers
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.