ArticleSkeletal muscle2011
Differentiation and fiber type-specific activity of a muscle creatine kinase intronic enhancer.
Article in Skeletal muscle, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
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The trial behind it
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Who cites it
23 citing papers in PubMed, 34 citations in OpenAlex.
- The exerkine apelin reverses age-associated sarcopenia.Nature medicine · 2018Trial
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- Hyaluronic Acid-Coated Melt Electrowritten Scaffolds Promote Myoblast Attachment, Alignment, and Differentiation.bioRxiv : the preprint server for biology · 2025Article
- REST/NRSF Preserves muscle stem cell identity by repressing alternate cell fate.Nature communications · 2025Article
- The Role of Insulin in the Proliferation and Differentiation of Bovine Muscle Satellite (Stem) Cells for Cultured Meat Production.International journal of molecular sciences · 2025Article
- Hexavalent chromium inhibits myogenic differentiation and induces myotube atrophy.Toxicology and applied pharmacology · 2023Article
- Integrated Comparative Transcriptome and circRNA-lncRNA-miRNA-mRNA ceRNA Regulatory Network Analyses Identify Molecular Mechanisms Associated with Intramuscular Fat Content in Beef Cattle.Animals : an open access journal from MDPI · 2023Article
- CKM and TERT dual promoters drive CRISPR-dCas9 to specifically inhibit the malignant behavior of osteosarcoma cells.Cellular & molecular biology letters · 2023Article
- Active vitamin D increases myogenic differentiation in C2C12 cells via a vitamin D response element on the myogenin promoter.Frontiers in physiology · 2023Article
- Creatine Kinase and Myoglobin Plasma Levels in Mountain Bike and Road Cyclists 1 h after the Race.International journal of environmental research and public health · 2022Article
- Application of ATAC-Seq for genome-wide analysis of the chromatin state at single myofiber resolution.eLife · 2022Article
- FOXP1 Interacts with MyoD to Repress its Transcription and Myoblast Conversion.Journal of cellular signaling · 2021Article
- A Muscle Hybrid Promoter as a Novel Tool for Gene Therapy.Molecular therapy. Methods & clinical development · 2019Article
- Sema3a-Nrp1 Signaling Mediates Fast-Twitch Myofiber Specificity of Tw2Developmental cell · 2019Article
- Requirement of Pitx2 for skeletal muscle homeostasis.Developmental biology · 2019Article
- Functional validation and expression analysis of myotubes converted from skin fibroblasts using a simple direct reprogramming strategy.eNeurologicalSci · 2017Article
- Nebulin deficiency in adult muscle causes sarcomere defects and muscle-type-dependent changes in trophicity: novel insights in nemaline myopathy.Human molecular genetics · 2015Article
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- TBX2 blocks myogenesis and promotes proliferation in rhabdomyosarcoma cells.International journal of cancer · 2014Article
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 1 country.
Funding
Abstract
backgroundHundreds of genes, including muscle creatine kinase (MCK), are differentially expressed in fast- and slow-twitch muscle fibers, but the fiber type-specific regulatory mechanisms are not well understood.
resultsModulatory region 1 (MR1) is a 1-kb regulatory region within MCK intron 1 that is highly active in terminally differentiating skeletal myocytes in vitro. A MCK small intronic enhancer (MCK-SIE) containing a paired E-box/myocyte enhancer factor 2 (MEF2) regulatory motif resides within MR1. The SIE's transcriptional activity equals that of the extensively characterized 206-bp MCK 5'-enhancer, but the MCK-SIE is flanked by regions that can repress its activity via the individual and combined effects of about 15 different but highly conserved 9- to 24-bp sequences. ChIP and ChIP-Seq analyses indicate that the SIE and the MCK 5'-enhancer are occupied by MyoD, myogenin and MEF2. Many other E-boxes located within or immediately adjacent to intron 1 are not occupied by MyoD or myogenin. Transgenic analysis of a 6.5-kb MCK genomic fragment containing the 5'-enhancer and proximal promoter plus the 3.2-kb intron 1, with and without MR1, indicates that MR1 is critical for MCK expression in slow- and intermediate-twitch muscle fibers (types I and IIa, respectively), but is not required for expression in fast-twitch muscle fibers (types IIb and IId).
conclusionsIn this study, we discovered that MR1 is critical for MCK expression in slow- and intermediate-twitch muscle fibers and that MR1's positive transcriptional activity depends on a paired E-box MEF2 site motif within a SIE. This is the first study to delineate the DNA controls for MCK expression in different skeletal muscle fiber types.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.