ArticleTissue engineering. Part C, Methods2011
Effects of hypertonic (NaCl) two-dimensional and three-dimensional culture conditions on the properties of cartilage tissue engineered from an expanded mature bovine chondrocyte source.
Article in Tissue engineering. Part C, Methods, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed.
- Biophysical Modulation of Mesenchymal Stem Cell Differentiation in the Context of Skeletal Repair.International journal of molecular sciences · 2022Review
- Nutrient Channels Aid the Growth of Articular Surface-Sized Engineered Cartilage Constructs.Tissue engineering. Part A · 2016Article
- Promoting increased mechanical properties of tissue engineered neocartilage via the application of hyperosmolarity and 4α-phorbol 12,13-didecanoate (4αPDD).Journal of biomechanics · 2014Article
- Effect of long-term osmotic loading culture on matrix synthesis from intervertebral disc cells.BioResearch open access · 2014Article
- Applied osmotic loading for promoting development of engineered cartilage.Journal of biomechanics · 2013Article
- Insulin, ascorbate, and glucose have a much greater influence than transferrin and selenous acid on the in vitro growth of engineered cartilage in chondrogenic media.Tissue engineering. Part A · 2013Article
- Mechanical regulation of chondrogenesis.Stem cell research & therapy · 2013Review
- Effect of cartilage endplate on cell based disc regeneration: a finite element analysis.Molecular & cellular biomechanics : MCB · 2013Article
- Trimethylamine N-oxide as a media supplement for cartilage tissue engineering.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2012Article
- Biomechanics-driven chondrogenesis: from embryo to adult.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2012Review
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Authors and funding
6 authors.
Funding
Abstract
Clinically relevant mature cartilage cells (chondrocytes) present challenges for use in cartilage tissue engineering applications, given their low capacity for cell division and tissue production. Since the in situ environment of chondrocytes is hypertonic relative to standard culture medium conditions, in this study we tested the hypothesis that using culture medium of a hypertonic, more physiologic osmolarity during both two-dimensional (2D) expansion of mature bovine chondrocytes (MBCs) and their subsequent encapsulation culture in three-dimensional (3D) agarose hydrogel constructs produces improved engineered tissue construct mechanical and biochemical properties. Results demonstrate that 2D expansion of MBCs in hypertonic (NaCl) medium before encapsulation yielded improved construct mechanical properties. However, 3D encapsulation culture of cells in hypertonic (NaCl) medium yielded poorer construct mechanical properties. Osmolarity-related differences in construct biochemical content and organization may have contributed to differences in mechanical properties, as construct glycosaminoglycan content correlated moderately with construct mechanical properties, and construct collagen distribution varied between 3D osmotic culture groups. Results of this study suggest that application of hypertonic (NaCl) medium during 2D mature chondrocyte expansion, but not 3D encapsulated chondrocyte culture, may serve as a convenient and inexpensive method for improving mechanical properties of expanded cell-seeded constructs.
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