ArticlePlanta2012
Functional analysis of OsPUT1, a rice polyamine uptake transporter.
Article in Planta, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 70 citations in OpenAlex.
- Dual functions of thermospermine in plant growth and stress responses.Amino acids · 2026Review
- The polyamine uptake transporters PUT2/LAT4 and PUT5/LAT5 contribute to Arabidopsis defense response againstPhysiology and molecular biology of plants : an international journal of functional plant biology · 2025Article
- HB52-PUT2 Module-Mediated Polyamine Shoot-to-Root Movement Regulates Salt Stress Tolerance in Tomato.Plant, cell & environment · 2025Article
- Paraquat resistance mutations have differential effects on plant fitness in two rice cultivars.The Biochemical journal · 2025Article
- Polyamines Interaction with Gaseous Signaling Molecules for Resilience Against Drought and Heat Stress in Plants.Plants (Basel, Switzerland) · 2025Review
- Polyamines: pleiotropic molecules regulating plant development and enhancing crop yield and quality.Plant biotechnology journal · 2024Review
- Polyamines in Plant-Pathogen Interactions: Roles in Defense Mechanisms and Pathogenicity with Applications in Fungicide Development.International journal of molecular sciences · 2024Review
- Suppression of pyrrolidine ring biosynthesis and its effects on gene expression and subsequent accumulation of anatabine in leaves of tobacco (N. tabacum L.).BMC genomics · 2023Article
- Genome-Wide Identification and Characterization of the Polyamine Uptake Transporter (Put) Gene Family in Tomatoes and the Role of Put2 in Response to Salt Stress.Antioxidants (Basel, Switzerland) · 2023Article
- Genetic portrait of polyamine transporters in barley: insights in the regulation of leaf senescence.Frontiers in plant science · 2023Article
- Disruption of three polyamine uptake transporter genes in rice by CRISPR/Cas9 gene editing confers tolerance to herbicide paraquat.aBIOTECH · 2022Article
- Differential Responses to Salt Stress in Four White Clover Genotypes Associated With Root Growth, Endogenous Polyamines Metabolism, and Sodium/Potassium Accumulation and Transport.Frontiers in plant science · 2022Article
- Overexpression of EiKCS confers paraquat-resistance in rice (Oryza sativa L.) by promoting the polyamine pathway.Pest management science · 2022Article
- Harnessing a Transient Gene Expression System inPlants (Basel, Switzerland) · 2021Article
- Biologia futura: the role of polyamine in plant science.Biologia futura · 2020Review
- The Spermine Synthase OsSPMS1 Regulates Seed Germination, Grain Size, and Yield.Plant physiology · 2018Article
- Molecular bases for differential aging programs between flag and second leaves during grain-filling in rice.Scientific reports · 2017Article
- Phloem-Specific Methionine Recycling Fuels Polyamine Biosynthesis in a Sulfur-Dependent Manner and Promotes Flower and Seed Development.Plant physiology · 2016Article
- Cadaverine's Functional Role in Plant Development and Environmental Response.Frontiers in plant science · 2016Review
- Metabolic and transcriptional transitions in barley glumes reveal a role as transitory resource buffers during endosperm filling.Journal of experimental botany · 2015Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Polyamines are nitrogenous compounds found in all eukaryotic and prokaryotic cells and absolutely essential for cell viability. In plants, they regulate several growth and developmental processes and the levels of polyamines are also correlated with the plant responses to various biotic and abiotic stresses. In plant cells, polyamines are synthesized in plastids and cytosol. This biosynthetic compartmentation indicates that the specific transporters are essential to transport polyamines between the cellular compartments. In the present study, a phylogenetic analysis was used to identify candidate polyamine transporters in rice. A full-length cDNA rice clone AK068055 was heterologously expressed in the Saccharomyces cerevisiae spermidine uptake mutant, agp2∆. Radiological uptake and competitive inhibition studies with putrescine indicated that rice gene encodes a protein that functioned as a spermidine-preferential transporter. In competition experiments with several amino acids at 25-fold higher levels than spermidine, only methionine, asparagine, and glutamine were effective in reducing uptake of spermidine to 60% of control rates. Based on those observations, this rice gene was named polyamine uptake transporter 1 (OsPUT1). Tissue-specific expression of OsPUT1 by semiquantitative RT-PCR showed that the gene was expressed in all tissues except seeds and roots. Transient expression assays in onion epidermal cells and rice protoplasts failed to localize to a cellular compartment. The characterization of the first plant polyamine transporter sets the stage for a systems approach that can be used to build a model to fully define how the biosynthesis, degradation, and transport of polyamines in plants mediate developmental and biotic responses.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.