ArticleActa crystallographica. Section F, Structural biology and crystallization communications2011
Cloning, expression, purification, crystallization and preliminary crystallographic analysis of MsDpo4: a Y-family DNA polymerase from Mycobacterium smegmatis.
Article in Acta crystallographica. Section F, Structural biology and crystallization communications, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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4 citing papers in PubMed, 5 citations in OpenAlex.
- A Quinoxaline 1,4-Dioxide Activates DNA Repair Systems inInternational journal of molecular sciences · 2025Article
- A polar filter in DNA polymerases prevents ribonucleotide incorporation.Nucleic acids research · 2019Article
- Pyrophosphate hydrolysis is an intrinsic and critical step of the DNA synthesis reaction.Nucleic acids research · 2018Article
- MsDpo4-a DinB Homolog from Mycobacterium smegmatis-Is an Error-Prone DNA Polymerase That Can Promote G:T and T:G Mismatches.Journal of nucleic acids · 2012Article
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2 authors at 1 institution in 1 country.
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Abstract
The expression of error-prone DNA polymerases belonging to the Y-family is upregulated in prokaryotes under adverse conditions to facilitate adaptive mutagenesis. However, it has been suggested that representatives of this family in mycobacteria do not participate in adaptive mutagenesis. These studies raise the possibility that mycobacterial representatives might be devoid of biochemical activity. In order to determine whether this possible loss of activity is a consequence of significant changes in the structure of these enzymes, structural studies on a representative enzyme from Mycobacterium smegmatis, MsDpo4, were initiated. The protein crystallized in space group P6(1)22 or P6(5)22. The Matthews coefficient was 4.0 Å3 Da(-1) assuming the presence of one molecule in the asymmetric unit; the corresponding solvent content was 69%. X-ray diffraction data were collected to a minimum Bragg spacing of 2.6 Å and crystals of selenomethionine-labelled MsDpo4 have been prepared for ab initio phasing.
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