ArticleThe Journal of biological chemistry2011
The assembly of proline-rich membrane anchor (PRiMA)-linked acetylcholinesterase enzyme: glycosylation is required for enzymatic activity but not for oligomerization.
Article in The Journal of biological chemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- Acetylcholinesterase: Structure, dynamics, and interactions with organophosphorus compounds.Protein science : a publication of the Protein Society · 2025Review
- The Tacrine-Induced Endoplasmic Reticulum Stress in AChE-Expressed Cells Leads to Improper Assembly and Transport of the Oligomeric Enzyme: Reversal by Trehalose.Journal of neurochemistry · 2025Article
- The Time Profile of the Effects of Moderate Hypothermia on Synaptic Acetylcholinesterase in Rat Brain.Bulletin of experimental biology and medicine · 2023Article
- An essential Noc3p dimerization cycle mediates ORC double-hexamer formation in replication licensing.Life science alliance · 2023Article
- Presenilin 1 Modulates Acetylcholinesterase Trafficking and Maturation.International journal of molecular sciences · 2023Article
- Proceedings of workshop: "Neuroglycoproteins in health and disease", INNOGLY cost action.Glycoconjugate journal · 2022Article
- Genome-Wide Methylation Mapping Using Nanopore Sequencing Technology Identifies Novel Tumor Suppressor Genes in Hepatocellular Carcinoma.International journal of molecular sciences · 2021Article
- Interacting withActa pharmaceutica Sinica. B · 2020Article
- Genistein, a Phytoestrogen in Soybean, Induces the Expression of Acetylcholinesterase via G Protein-Coupled Receptor 30 in PC12 Cells.Frontiers in molecular neuroscience · 2018Article
- Biogenesis, assembly and trafficking of acetylcholinesterase.Journal of neurochemistry · 2017Review
- Wnt3a induces the expression of acetylcholinesterase during osteoblast differentiation via the Runx2 transcription factor.The Journal of biological chemistry · 2017Article
- LW-AFC Effects onAging and disease · 2017Article
- Three N-Glycosylation Sites of Human Acetylcholinesterase Shares Similar Glycan Composition.Journal of molecular neuroscience : MN · 2015Article
- Reduced Expression of P2Y2 Receptor and Acetylcholinesterase at Neuromuscular Junction of P2Y1 Receptor Knock-out Mice.Journal of molecular neuroscience : MN · 2015Article
- Relative Quantification and Higher-Order Modeling of the Plasma Glycan Cancer Burden Ratio in Ovarian Cancer Case-Control Samples.Journal of proteome research · 2015Article
- Rescue and Stabilization of Acetylcholinesterase in Skeletal Muscle by N-terminal Peptides Derived from the Noncatalytic Subunits.The Journal of biological chemistry · 2015Article
- Definitive Screening Design Optimization of Mass Spectrometry Parameters for Sensitive Comparison of Filter and Solid Phase Extraction Purified, INLIGHT Plasma N-Glycans.Analytical chemistry · 2015Article
- Quantification of the transcripts encoding different forms of AChE in various cell types: real-time PCR coupled with standards in revealing the copy number.Journal of molecular neuroscience : MN · 2014Article
- Most acetylcholinesterase activity of non-nervous tissues and cells arises from the AChE-H transcript.Journal of molecular neuroscience : MN · 2014Article
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8 authors.
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Abstract
Acetylcholinesterase (AChE) anchors onto cell membranes by a transmembrane protein PRiMA (proline-rich membrane anchor) as a tetrameric form in vertebrate brain. The assembly of AChE tetramer with PRiMA requires the C-terminal "t-peptide" in AChE catalytic subunit (AChE(T)). Although mature AChE is well known N-glycosylated, the role of glycosylation in forming the physiologically active PRiMA-linked AChE tetramer has not been studied. Here, several lines of evidence indicate that the N-linked glycosylation of AChE(T) plays a major role for acquisition of AChE full enzymatic activity but does not affect its oligomerization. The expression of the AChE(T) mutant, in which all N-glycosylation sites were deleted, together with PRiMA in HEK293T cells produced a glycan-depleted PRiMA-linked AChE tetramer but with a much higher K(m) value as compared with the wild type. This glycan-depleted enzyme was assembled in endoplasmic reticulum but was not transported to Golgi apparatus or plasma membrane.
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