Evidence map›Paper›PMID 21795509›Full record

ArticleJournal of clinical microbiology2011

Assessment of two new molecular methods for identification of Candida parapsilosis sensu lato species.

Guillermo Garcia-Effron, Emilia Canton, Javier Pemán, Amanda Dilger, Eva Romá, David S Perlin

Abstract readEvaluation Study
In one paragraph

Article in Journal of clinical microbiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.

0numbers the graph read from it
0cells of the map it votes in
16citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

16 citing papers in PubMed.

  1. The Emerging PathogenInfection and drug resistance · 2024
    Review
  2. Review
  3. Review
  4. Article
  5. Sequencing ofAntimicrobial agents and chemotherapy · 2018
    Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Guillermo Garcia-EffronPublic Health Research Institute, New Jersey Medical School—UMDNJ, Newark, New Jersey, USA.
Emilia Canton
Javier Pemán
Amanda Dilger
Eva Romá
David S Perlin

Funding

Mechanism of clinical resistance to echinocandin antifungal drugsR01AI069397 · NIAID · UNIV OF MED/DENT OF NJ-NJ MEDICAL SCHOOL · PI PERLIN, DAVID S · 2007 to 2011
$2.0M
NIAID NIH HHS AI069397NIAID NIH HHS R01 AI069397
6 · The paper itself

Abstract

Candida parapsilosis sensu stricto, C. orthopsilosis, and C. metapsilosis replaced C. parapsilosis groups I, II, and III in 2005. Since then, an increased interest in studying their epidemiology has arisen based on the observed differences in antifungal susceptibilities and virulence the three species. A strict differentiation of these species cannot be achieved by phenotypic methods. We evaluate two new molecular methodologies to differentiate among these species by the use of a collection of 293 bloodstream infection isolates of C. parapsilosis sensu lato. For the first method, the isolates were studied using PCR amplification of a fragment of the C. parapsilosis sensu lato FKS1 gene and a universal primer pair followed by EcoRI enzyme digestion. The other method used the allele discrimination ability of molecular beacons in a multiplex real-time PCR format. Both methods of identification showed 100% concordance with internal transcribed spacer 1 (ITS1)/ITS2 sequencing and proved to be effective for clinical applications, even with mixed-species DNAs.

Indexed as

CandidaCandidemiaDNA, FungalDNA PrimersDNA, Ribosomal SpacerFungal ProteinsHumansMolecular Diagnostic TechniquesMolecular Sequence DataMycologyPolymerase Chain ReactionSequence Analysis, DNADNA, FungalDNA PrimersDNA, Ribosomal SpacerFungal Proteins

Identifiers

PMID21795509
PMCPMC3165596

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.