ArticleMolecular biology of the cell2011
Evidence for a fence that impedes the diffusion of phosphatidylinositol 4,5-bisphosphate out of the forming phagosomes of macrophages.
Article in Molecular biology of the cell, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
47 citing papers in PubMed, 75 citations in OpenAlex.
- Inhibitory Fc Receptor sets a time limit on macrophage response to IgG.bioRxiv : the preprint server for biology · 2026Article
- Formation of Membrane Domains via Actin Waves: A Fundamental Principle in the Generation of Dynamic Structures in Phagocytes.International journal of molecular sciences · 2025Review
- Synthetic Lipid Biology.Chemical reviews · 2025Review
- Review
- PI(4,5)P2 diffuses freely in the plasma membrane even within high-density effector protein complexes.The Journal of cell biology · 2023Article
- Localisation of Intracellular Signals and Responses during Phagocytosis.International journal of molecular sciences · 2023Review
- PIPBiology · 2022Article
- Quantitative FRET Microscopy Reveals a Crucial Role of Cytoskeleton in Promoting PI(4,5)PInternational journal of molecular sciences · 2021Article
- Compartmentalization of phosphatidylinositol 4,5-bisphosphate metabolism into plasma membrane liquid-ordered/raft domains.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Imaging FCS delineates subtle heterogeneity in plasma membranes of resting mast cells.Molecular biology of the cell · 2020Article
- Characterization of Specific Ion Effects on PI(4,5)PThe journal of physical chemistry. B · 2020Article
- Permeability of membranes in the liquid ordered and liquid disordered phases.Nature communications · 2019Article
- Stochastic geometry sensing and polarization in a lipid kinase-phosphatase competitive reaction.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Influenza Hemagglutinin Modulates Phosphatidylinositol 4,5-Bisphosphate Membrane Clustering.Biophysical journal · 2019Article
- The origins and evolution of macropinocytosis.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2019Review
- Visualization of expanding fusion pores in secretory cells.The Journal of general physiology · 2018Review
- High expression of type I inositol 1,4,5-trisphosphate receptor in the kidney of rats with hepatorenal syndrome.World journal of gastroenterology · 2018Article
- Phosphoinositide 3-kinase pathways and autophagy require phosphatidylinositol phosphate kinases.Advances in biological regulation · 2018Review
- The Sealing Zone in Osteoclasts: A Self-Organized Structure on the Bone.International journal of molecular sciences · 2018Review
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 6 institutions in 3 countries.
Funding
Abstract
To account for the many functions of phosphatidylinositol 4,5-bisphosphate (PIP(2)), several investigators have proposed that there are separate pools of PIP(2) in the plasma membrane. Recent experiments show the surface concentration of PIP(2) is indeed enhanced in regions where phagocytosis, exocytosis, and cell division occurs. Kinases that produce PIP(2) are also concentrated in these regions. However, how is the PIP(2) produced by these kinases prevented from diffusing rapidly away? First, proteins could act as "fences" around the perimeter of these regions. Second, some factor could markedly decrease the diffusion coefficient, D, of PIP(2) within these regions. We used fluorescence correlation spectroscopy (FCS) and fluorescence recovery after photobleaching (FRAP) to investigate these two possibilities in the forming phagosomes of macrophages injected with fluorescent PIP(2). FCS measurements show that PIP(2) diffuses rapidly (D ~ 1 μm(2)/s) in both the forming phagosomes and unengaged plasma membrane. FRAP measurements show that the fluorescence from PIP(2) does not recover (>100 s) after photobleaching the entire forming phagosome but recovers rapidly (~10 s) in a comparable area of membrane outside the cup. These results (and similar data for a plasma membrane-anchored green fluorescent protein) support the hypothesis that a fence impedes the diffusion of PIP(2) into and out of forming phagosomes.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.