ArticleMolecular biology of the cell2011
An active kinase domain is required for retention of PKCθ at the T cell immunological synapse.
Article in Molecular biology of the cell, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 18 citations in OpenAlex.
- The Emerging Function of PKCtheta in Cancer.Biomolecules · 2021Review
- Phosphotyrosine-dependent interaction between the kinases PKCθ and Zap70 promotes proximal TCR signaling.Science signaling · 2019Article
- FDA-approved immunosuppressants targeting staphylococcal superantigens: mechanisms and insights.ImmunoTargets and therapy · 2017Review
- Intracellular Delivery of Anti-pPKCθ (Thr538) via Protein Transduction Domain Mimics for Immunomodulation.Molecular therapy : the journal of the American Society of Gene Therapy · 2016Article
- Cell Type-Specific Regulation of Immunological Synapse Dynamics by B7 Ligand Recognition.Frontiers in immunology · 2016Review
- Staphylococcal Superantigens Spark Host-Mediated Danger Signals.Frontiers in immunology · 2016Review
- TCR-induced sumoylation of the kinase PKC-θ controls T cell synapse organization and T cell activation.Nature immunology · 2015Article
- The Novel PKCθ from Benchtop to Clinic.Journal of immunology research · 2015Review
- Lipid-based patterning of the immunological synapse.Biochemical Society transactions · 2014Review
- The variable hinge region of novel PKCs determines localization to distinct regions of the immunological synapse.PloS one · 2014Article
- Update on staphylococcal superantigen-induced signaling pathways and therapeutic interventions.Toxins · 2013Review
- A new look at T cell receptor signaling to nuclear factor-κB.Trends in immunology · 2013Review
- In and out of the bull's eye: protein kinase Cs in the immunological synapse.Trends in immunology · 2013Review
- The yin and yang of protein kinase C-theta (PKCθ): a novel drug target for selective immunosuppression.Advances in pharmacology (San Diego, Calif.) · 2013Review
- PI3K/Akt/mTOR, a pathway less recognized for staphylococcal superantigen-induced toxicity.Toxins · 2012Review
- Intervention of PKC-θ as an immunosuppressive regimen.Frontiers in immunology · 2012Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Protein kinase Cθ (PKCθ) is a serine/threonine kinase that plays an essential role in antigen-regulated responses of T lymphocytes. Upon antigen stimulation, PKCθ is rapidly recruited to the immunological synapse (IS), the region of contact between the T cell and antigen-presenting cell. This behavior is unique among T cell PKC isoforms. To define domains of PKCθ required for retention at the IS, we generated deletion and point mutants of PKCθ. We used quantitative imaging analysis to assess IS retention of PKCθ mutants in antigen-stimulated T cell clones. Deletion of the kinase domain or site-directed mutation of a subset of known PKCθ phosphorylation sites abrogated or significantly reduced IS retention, respectively. IS retention did not correlate with phosphorylation of specific PKCθ residues but rather with kinase function. Thus PKCθ catalytic competence is essential for stable IS retention.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.