ArticleGenome research2011
A proteogenomic analysis of Anopheles gambiae using high-resolution Fourier transform mass spectrometry.
Article in Genome research, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed.
- TheProceedings of the National Academy of Sciences of the United States of America · 2025Article
- A Proteogenomic Approach for the Identification of Virulence Factors in Leishmania Parasites.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Comparative Proteogenomic Approaches for Mapping the Global Proteome of the Unsequenced Leishmania Vector Phlebotomus papatasi.Methods in molecular biology (Clifton, N.J.) · 2025Article
- PgxSAVy: A tool for comprehensive evaluation of variant peptide quality in proteogenomics - catching the (un)usual suspects.Computational and structural biotechnology journal · 2024Article
- Peptides with Antimicrobial Activity in the Saliva of the Malaria VectorInternational journal of molecular sciences · 2024Article
- Malaria: influence of Anopheles mosquito saliva on Plasmodium infection.Trends in immunology · 2023Review
- Article
- Proteomics reveals localization of cuticular proteins in Anopheles gambiae.Insect biochemistry and molecular biology · 2019Article
- Unraveling the Plasmodium vivax sporozoite transcriptional journey from mosquito vector to human host.Scientific reports · 2018Article
- Integrated Multi-Omic Analysis ofFrontiers in microbiology · 2018Article
- Retention of duplicated long-wavelength opsins in mosquito lineages by positive selection and differential expression.BMC evolutionary biology · 2017Article
- Secretome analysis of diarrhea-inducing strains of Escherichia coli.Proteomics · 2017Article
- Anopheline salivary protein genes and gene families: an evolutionary overview after the whole genome sequence of sixteen Anopheles species.BMC genomics · 2017Article
- Integrating transcriptomic and proteomic data for accurate assembly and annotation of genomes.Genome research · 2017Article
- GAPP: A Proteogenomic Software for Genome Annotation and Global Profiling of Post-translational Modifications in Prokaryotes.Molecular & cellular proteomics : MCP · 2016Article
- Identification of GAPDH on the surface of Plasmodium sporozoites as a new candidate for targeting malaria liver invasion.The Journal of experimental medicine · 2016Article
- Distribution of cuticular proteins in different structures of adult Anopheles gambiae.Insect biochemistry and molecular biology · 2016Article
- Tissue-specific Proteogenomic Analysis of Plutella xylostella Larval Midgut Using a Multialgorithm Pipeline.Molecular & cellular proteomics : MCP · 2016Article
- "Salivary gland cellular architecture in the Asian malaria vector mosquito Anopheles stephensi".Parasites & vectors · 2015Article
- A multi-omic analysis of human naïve CD4+ T cells.BMC systems biology · 2015Article
Corrections and comments
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25 authors.
Funding
Abstract
Anopheles gambiae is a major mosquito vector responsible for malaria transmission, whose genome sequence was reported in 2002. Genome annotation is a continuing effort, and many of the approximately 13,000 genes listed in VectorBase for Anopheles gambiae are predictions that have still not been validated by any other method. To identify protein-coding genes of An. gambiae based on its genomic sequence, we carried out a deep proteomic analysis using high-resolution Fourier transform mass spectrometry for both precursor and fragment ions. Based on peptide evidence, we were able to support or correct more than 6000 gene annotations including 80 novel gene structures and about 500 translational start sites. An additional validation by RT-PCR and cDNA sequencing was successfully performed for 105 selected genes. Our proteogenomic analysis led to the identification of 2682 genome search-specific peptides. Numerous cases of encoded proteins were documented in regions annotated as intergenic, introns, or untranslated regions. Using a database created to contain potential splice sites, we also identified 35 novel splice junctions. This is a first report to annotate the An. gambiae genome using high-accuracy mass spectrometry data as a complementary technology for genome annotation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.