ArticleThe international journal of biochemistry & cell biology2011
Drosha regulates hMSCs cell cycle progression through a miRNA independent mechanism.
Article in The international journal of biochemistry & cell biology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
17 citing papers in PubMed, 20 citations in OpenAlex.
- Downregulation ofInternational journal of molecular sciences · 2023Article
- DROSHA but not DICER is required for human haematopoietic stem cell function.Clinical & translational immunology · 2022Article
- Control of ribosomal protein synthesis by the Microprocessor complex.Science signaling · 2021Article
- Loss of Drosha underlies dopaminergic neuron toxicity in models of Parkinson's disease.Cell death & disease · 2018Article
- Cell Cycle Regulation of Stem Cells by MicroRNAs.Stem cell reviews and reports · 2018Review
- Polycomb group protein Suz12 is regulated by a novel miRNA-like small RNA.Scientific reports · 2018Article
- Double-edged sword of mesenchymal stem cells: Cancer-promoting versus therapeutic potential.Cancer science · 2017Review
- A network-biology perspective of microRNA function and dysfunction in cancer.Nature reviews. Genetics · 2016Review
- Review
- AKI Recovery Induced by Mesenchymal Stromal Cell-Derived Extracellular Vesicles Carrying MicroRNAs.Journal of the American Society of Nephrology : JASN · 2015Article
- MicroRNA-103a-3p controls proliferation and osteogenic differentiation of human adipose tissue-derived stromal cells.Experimental & molecular medicine · 2015Article
- Stress induces p38 MAPK-mediated phosphorylation and inhibition of Drosha-dependent cell survival.Molecular cell · 2015Article
- The ARF tumor-suppressor controls Drosha translation to prevent Ras-driven transformation.Oncogene · 2014Article
- MicroRNA Machinery Genes as Novel Biomarkers for Cancer.Frontiers in oncology · 2014Review
- MicroRNA-independent roles of the RNase III enzymes Drosha and Dicer.Open biology · 2013Review
- Expression of the RNase III enzyme DROSHA is reduced during progression of human cutaneous melanoma.Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc · 2013Article
- Mesenchymal stem cells in tumor development: emerging roles and concepts.Cell adhesion & migrationReview
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Recently we demonstrated that the miRNA regulate human mesenchymal stem cells (hMSCs) differentiation. To determine the role of the miRNA pathway in hMSCs proliferation, Drosha and Dicer knockdown hMSCs were generated using a lentiviral based tetracycline inducible shRNA. hMSCs with reduced Drosha expression had a significantly reduced proliferation rate, while hMSCs with reduced Dicer expression displayed a proliferation rate similar to untransduced cells. Cell cycle analysis identified that unlike Dicer knockdown, Drosha knockdown hMSCs contained an increased number of G1 phase cells, with a reduced level of cells in S phase, compared to controls. ELISAs of hMSCs revealed decreased levels of pRB and stable levels of total RB with Drosha knockdown. Two key regulators of the G1/S phase transition, cyclin dependent kinase inhibitor 2A (p16) and cyclin dependent kinase inhibitor 2B (p15), were increased in Drosha knockdown cells but not in Dicer knockdown. Transcripts of 28S and 18S rRNA were significantly reduced in Drosha knockdown hMSCs, with no change in rRNA levels in Dicer knockdown hMSCs. 45S pre-rRNA transcripts were not significantly different in either knockdown model. The above results indicate that Drosha modifies hMSCs proliferation through a miRNA independent mechanism, potentially by regulating rRNA processing.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.