ArticleProteome science2011
Identification of azurocidin as a potential periodontitis biomarker by a proteomic analysis of gingival crevicular fluid.
Article in Proteome science, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers, 1 of them a synthesis that pooled it.
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Who cites it
27 citing papers in PubMed, 1 synthesis or guideline pooled it, 70 citations in OpenAlex.
- MS-Based Crevicular Fluid Proteomics for the Study of Periodontal and Peri-Implant Conditions: A Systematic Review.Journal of proteome research · 2026Pooled it
- The Role of the Oral Microbiome in the Development and Treatment of Periodontal Disease: Functional Meta-Omics Evidence.Current microbiology · 2026Review
- Evaluation on the significance of miR-199a-5p for preterm premature rupture of membranes with chorioamnionitis.BMC pregnancy and childbirth · 2026Article
- Proteomic Analysis of the Periodontal Ligament During Orthodontic Movement: A Study in Rats.Proteomes · 2025Article
- Diagnostic value of aMMP-8 and azurocidin in peri-implant sulcular fluid as biomarkers of peri-implant health or disease.Clinical and experimental dental research · 2024Article
- Alpha-Defensin 1: An Emerging Periodontitis Biomarker.Diagnostics (Basel, Switzerland) · 2023Article
- Article
- Proteomic analysis of sialoliths from calcified, lipid and mixed groups as a source of potential biomarkers of deposit formation in the salivary glands.Clinical proteomics · 2023Article
- Potential Impact of Prosthetic Biomaterials on the Periodontium: A Comprehensive Review.Molecules (Basel, Switzerland) · 2023Review
- Proteomics Disclose the Potential of Gingival Crevicular Fluid (GCF) as a Source of Biomarkers for Severe Periodontitis.Materials (Basel, Switzerland) · 2022Article
- Dental, oral pH, orthodontic and salivary values in children with obstructive sleep apnea.Clinical oral investigations · 2022Article
- Future Drug Targets in Periodontal Personalised Medicine-A Narrative Review.Journal of personalized medicine · 2022Review
- Comparative antler proteome of sika deer from different developmental stages.Scientific reports · 2021Article
- Characterization of Extracellular Vesicle Cargo in Sjögren's Syndrome through a SWATH-MS Proteomics Approach.International journal of molecular sciences · 2021Article
- Gingival Crevicular Fluid Peptidome Profiling in Healthy and in Periodontal Diseases.International journal of molecular sciences · 2020Review
- Review
- Host-microbe interactions: Profiles in the transcriptome, the proteome, and the metabolome.Periodontology 2000 · 2020Review
- Role of Calprotectin as a Biomarker in Periodontal Disease.Mediators of inflammation · 2019Review
- Current Status of Proteomic Technologies for Discovering and Identifying Gingival Crevicular Fluid Biomarkers for Periodontal Disease.International journal of molecular sciences · 2018Review
- High-throughput proteomic analysis of candidate biomarker changes in gingival crevicular fluid after treatment of chronic periodontitis.Journal of periodontal research · 2018Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe inflammatory disease periodontitis results in tooth loss and can even lead to diseases of the whole body if not treated. Gingival crevicular fluid (GCF) reflects the condition of the gingiva and contains proteins transuded from serum or cells at inflamed sites. In this study, we aimed to discover potential protein biomarkers for periodontitis in GCF proteome using LC-MS/MS.
resultsWe identified 305 proteins from GCF of healthy individuals and periodontitis patients collected using a sterile gel loading tip by ESI-MS/MS coupled to nano-LC. Among these proteins, about 45 proteins were differentially expressed in the GCF proteome of moderate periodontitis patients when compared to the healthy individuals. We first identified azurocidin in the GCF, but not the saliva, as an upregulated protein in the periodontitis patients and verified its increased expression during periodontitis by ELISA using the GCF of the classified periodontitis patients compared to the healthy individuals. In addition, we found that azurocidin inhibited the differentiation of bone marrow-derived macrophages to osteoclasts.
conclusionsOur results show that GCF collection using a gel loading tip and subsequent LC-MS/MS analysis following 1D-PAGE proteomic separation are effective for the analysis of the GCF proteome. Our current results also suggest that azurocidin could be a potential biomarker candidate for the early detection of inflammatory periodontal destruction by gingivitis and some chronic periodontitis. Our data also suggest that azurocidin may have an inhibitory role in osteoclast differentiation and, thus, a protective role in alveolar bone loss during the early stages of periodontitis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.