ArticlePlant methods2011
Reverse genetics in Chlamydomonas: a platform for isolating insertional mutants.
Article in Plant methods, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
38 citing papers in PubMed, 99 citations in OpenAlex.
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- Cell Synchronization Enhances Nuclear Transformation and Genome EditingACS synthetic biology · 2020Article
- CrABCA2 Facilitates Triacylglycerol Accumulation inMolecules and cells · 2020Article
- Validation of a New Multicistronic Plasmid for the Efficient and Stable Expression of Transgenes in Microalgae.International journal of molecular sciences · 2020Article
- Good News for Nuclear Transgene Expression inCells · 2019Review
- Lipid remodeling regulator 1 (LRL1) is differently involved in the phosphorus-depletion response from PSR1 in Chlamydomonas reinhardtii.The Plant journal : for cell and molecular biology · 2019Article
- Article
- A Plant Cryptochrome Controls Key Features of thePlant physiology · 2017Article
- Building a multipurpose insertional mutant library for forward and reverse genetics inPlant methods · 2017Article
- Reverse Genetics and High Throughput Sequencing Methodologies for Plant Functional Genomics.Current genomics · 2016Article
- Calredoxin represents a novel type of calcium-dependent sensor-responder connected to redox regulation in the chloroplast.Nature communications · 2016Article
- An Indexed, Mapped Mutant Library Enables Reverse Genetics Studies of Biological Processes in Chlamydomonas reinhardtii.The Plant cell · 2016Article
- Critical role of Chlamydomonas reinhardtii ferredoxin-5 in maintaining membrane structure and dark metabolism.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Dissecting the contributions of GC content and codon usage to gene expression in the model alga Chlamydomonas reinhardtii.The Plant journal : for cell and molecular biology · 2015Article
- Chlamydomonas Genome Resource for Laboratory Strains Reveals a Mosaic of Sequence Variation, Identifies True Strain Histories, and Enables Strain-Specific Studies.The Plant cell · 2015Article
- Characterization of cooperative bicarbonate uptake into chloroplast stroma in the green alga Chlamydomonas reinhardtii.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Altered lipid composition and enhanced lipid production in green microalga by introduction of brassica diacylglycerol acyltransferase 2.Plant biotechnology journal · 2015Article
- Alternative acetate production pathways in Chlamydomonas reinhardtii during dark anoxia and the dominant role of chloroplasts in fermentative acetate production.The Plant cell · 2014Article
- The Chlamydomonas genome project: a decade on.Trends in plant science · 2014Review
- The carbon concentrating mechanism in Chlamydomonas reinhardtii: finding the missing pieces.Photosynthesis research · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
12 authors at 7 institutions in 5 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A method was developed to identify insertional mutants of Chlamydomonas reinhardtii disrupted for selected target genes. The approach relies on the generation of thousands of transformants followed by PCR-based screenings that allow for identification of strains harboring the introduced marker gene within specific genes of interest. Our results highlight the strengths and limitations of two independent screens that differed in the nature of the marker DNA used (PCR-amplified fragment containing the plasmid-free marker versus entire linearized plasmid with the marker) and in the strategies used to maintain and store transformants.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.