Evidence map›Paper›PMID 21794143›Full record

ArticleBMC microbiology2011

A multi locus variable number of tandem repeat analysis (MLVA) scheme for Streptococcus agalactiae genotyping.

Eve Haguenoer, Gaelle Baty, Christine Pourcel, Marie-Frédérique Lartigue, Anne-Sophie Domelier, Agnès Rosenau, Roland Quentin, Laurent Mereghetti, Philippe Lanotte

Open access · goldAbstract readEvaluation Study
In one paragraph

Article in BMC microbiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.

0numbers the graph read from it
0cells of the map it votes in
23citing papers in PubMed
5.0field-weighted citation impact, top 5% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

23 citing papers in PubMed, 43 citations in OpenAlex.

  1. Review
  2. Article
  3. Molecular epidemiology and antimicrobial resistance patterns of carbapenem-resistantFrontiers in cellular and infection microbiology · 2023
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  11. High Incidence of PathogenicFrontiers in microbiology · 2018
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 4 institutions in 1 country.

Eve HaguenoerUniversité François-Rabelais de Tours, UFR de Médecine, EA 3854 Bactéries et risque materno-fœtal, Institut Fédératif de Recherche 136 Agents Transmissibles et Infectiologie, Tours, France.
Gaelle Baty
Christine Pourcel
Marie-Frédérique Lartigue
Anne-Sophie Domelier
Agnès Rosenau
Roland Quentin
Laurent Mereghetti
Philippe Lanotte
Centre Hospitalier Universitaire de Tours · FRInstitut de Virologie · FRUniversité de Tours · FRUniversité Paris-Sud · FR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundMultilocus sequence typing (MLST) is currently the reference method for genotyping Streptococcus agalactiae strains, the leading cause of infectious disease in newborns and a major cause of disease in immunocompromised children and adults. We describe here a genotyping method based on multiple locus variable number of tandem repeat (VNTR) analysis (MLVA) applied to a population of S. agalactiae strains of various origins characterized by MLST and serotyping.

resultsWe studied a collection of 186 strains isolated from humans and cattle and three reference strains (A909, NEM316 and 2603 V/R). Among 34 VNTRs, 6 polymorphic VNTRs loci were selected for use in genotyping of the bacterial population. The MLVA profile consists of a series of allele numbers, corresponding to the number of repeats at each VNTR locus. 98 MLVA genotypes were obtained compared to 51 sequences types generated by MLST. The MLVA scheme generated clusters which corresponded well to the main clonal complexes obtained by MLST. However it provided a higher discriminatory power. The diversity index obtained with MLVA was 0.960 compared to 0.881 with MLST for this population of strains.

conclusionsThe MLVA scheme proposed here is a rapid, cheap and easy genotyping method generating results suitable for exchange and comparison between different laboratories and for the epidemiologic surveillance of S. agalactiae and analyses of outbreaks.

Indexed as

Minisatellite RepeatsAdultAnimalsCattleCattle DiseasesChildChild, PreschoolCluster AnalysisEpidemiologic MethodsGenotypeHumansInfant, NewbornMolecular TypingPolymorphism, GeneticSensitivity and SpecificityStreptococcal Infections

Identifiers

PMID21794143
PMCPMC3163538
OpenAlexW2018603569

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.