ArticleJournal of applied microbiology2011
Rapid and sensitive detection of Pseudomonas aeruginosa in chlorinated water and aerosols targeting gyrB gene using real-time PCR.
Article in Journal of applied microbiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers, 1 of them a synthesis that pooled it.
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Who cites it
28 citing papers in PubMed, 1 synthesis or guideline pooled it, 64 citations in OpenAlex.
- A Systematic Review of Culture-Based Methods for Monitoring Antibiotic-Resistant Acinetobacter, Aeromonas, and Pseudomonas as Environmentally Relevant Pathogens in Wastewater and Surface Water.Current environmental health reports · 2023Pooled it
- Comparison of the expression of small regulatory RNAs AS1974, AS2779, IGR2780 and the Hfq gene in carbapenem-resistant and susceptible clinical isolates of Pseudomonas aeruginosa.BMC microbiology · 2026Article
- Rapid and modular workflows for same-day sequencing-based detection of bloodstream infections and antimicrobial resistance determinants using culture-enriched samples.Microbiology spectrum · 2026Article
- Reproducibility crisis in isothermal amplification: lessons from benchmarking LAMP assays.Microbiology spectrum · 2026Article
- Microbiological Analysis Following Periodontal Treatment in Individuals with Bronchiectasis.Microorganisms · 2026Article
- Recombinase Polymerase Amplification (RPA) as an Isothermal Molecular-Point of Care Test (IM-POCT) for Rapid Identification of P. aeruginosa Among Patients with Respiratory Infection.Molecular biotechnology · 2026Article
- Understanding the Burden and Management of Urinary Tract Infections in Women.Diseases (Basel, Switzerland) · 2025Review
- Microbial risks in drinking water systems: persistence and public health implications of opportunistic premise plumbing pathogens.Frontiers in microbiology · 2025Article
- An Evaluation of the Sensitivity and Applicability of a Droplet Digital Polymerase Chain Reaction Assay to Simultaneously DetectFoods (Basel, Switzerland) · 2024Article
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- Development of a multiplex Loop-Mediated Isothermal Amplification (LAMP) for the diagnosis of bacterial periprosthetic joint infection.PloS one · 2024Article
- Evaluation of Sensitivity and Cost-Effectiveness of Molecular Methods for the Co-detection of Waterborne Pathogens in India.Marine biotechnology (New York, N.Y.) · 2021Article
- Bacteria Associated with Healthcare-Associated Infections on Environmental Samples Obtained from Two Fire Departments.International journal of environmental research and public health · 2021Article
- High fluoride resistance and virulence profile of environmental Pseudomonas isolated from water sources.Folia microbiologica · 2021Article
- Elucidating the Efficacy of Vaccination against Vibriosis inVaccines · 2020Article
- Simple, Rapid and Sensitive Detection of Pseudomonas aeruginesa by Colorimetric Multiple Cross Displacement Amplification.Current medical science · 2020Article
- Real-time polymerase chain reaction assays for rapid detection and virulence evaluation of the environmental Pseudomonas aeruginosa isolates.Molecular biology reports · 2019Article
- Propidium monoazide-quantitative polymerase chain reaction (PMA-qPCR) assay for rapid detection of viable and viable but non-culturable (VBNC)Journal of environmental health science & engineering · 2019Article
- Detection of pathogenic microorganisms from bloodstream infection specimens using TaqMan array card technology.Scientific reports · 2018Article
- Hospital Drains as Reservoirs of Pseudomonas aeruginosa: Multiple-Locus Variable-Number of Tandem Repeats Analysis Genotypes Recovered from Faucets, Sink Surfaces and Patients.Pathogens (Basel, Switzerland) · 2017Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
aimsFor the rapid detection of Pseudomonas aeruginosa from chlorinated water and aerosols, gyrB gene-based real-time PCR assay was developed and investigated. METHODS AND
resultsTwo novel primer sets (pa722F/746MGB/899R and pa722F/746MGB/788R) were designed using the most updated 611 Pseudomonas and 748 other bacterial gyrB genes for achieving high specificity. Their specificity showed 100% accuracy when tested with various strains including clinical isolates from cystic fibrosis patients. The assay was tested with Ps. aeruginosa-containing chlorinated water and aerosols to simulate the waterborne and airborne transmission routes (detection limit 3·3 × 10² CFU per PCR-2·3 × 10³ CFU per PCR). No chlorine interference in real-time PCR was observed at drinking water level (c. 1 mg l⁻¹), but high level of chorine (12 mg l⁻¹) interfered the assay, and thus neutralization was needed. Pseudomonas aeruginosa in aerosol was successfully detected after capturing with gelatin filters with minimum 2 min of sampling time when the initial concentration of 10⁴ CFU ml⁻¹ bacteria existed in the nebulizer.
conclusionsA highly specific and rapid assay (2-3 h) was developed by targeting gyrB gene for the detection of Ps. aeruginosa in chlorinated water and aerosols, combined with optimized sample collection methods and sample processing, so the direct DNA extraction from either water or aerosol was possible while achieving the desired sensitivity of the method. SIGNIFICANCE AND IMPACT OF THE STUDY: The new assay can provide timely and accurate risk assessment to prevent Ps. aeruginosa exposure from water and aerosol, resulting in reduced disease burden, especially among immune-compromised and susceptible individuals. This approach can be easily utilized as a platform technology for the detection of other types of micro-organisms, especially for those that are transmitted via water and aerosol routes, such as Legionella pneumophila.
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Registered trials
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