ArticlePancreas2012
Simplified method to isolate highly pure canine pancreatic islets.
Article in Pancreas, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- Isolation of feline islets of Langerhans by selective osmotic shock produces glucose responsive islets.Frontiers in veterinary science · 2024Article
- PEGDA microencapsulated allogeneic islets reverse canine diabetes without immunosuppression.PloS one · 2022Article
- Production, Characterization, and Function of Pseudoislets from Perinatal Canine Pancreas.Cell transplantation · 2019Article
- Article
- The Flaws and Future of Islet Volume Measurements.Cell transplantation · 2018Review
- Selective Osmotic Shock for Islet Isolation in the Cadaveric Canine Pancreas.Cell transplantation · 2018Article
- Improved yield of canine islet isolation from deceased donors.BMC veterinary research · 2017Article
- Durable Control of Autoimmune Diabetes in Mice Achieved by Intraperitoneal Transplantation of "Neo-Islets," Three-Dimensional Aggregates of Allogeneic Islet and "Mesenchymal Stem Cells".Stem cells translational medicine · 2017Article
- An optimized protocol for purification of functional islets of Langerhans.Laboratory investigation; a journal of technical methods and pathology · 2017Article
- Effective endothelial cell and human pluripotent stem cell interactions generate functional insulin-producing beta cells.Diabetologia · 2016Article
- Exenatide Treatment Alone Improves β-Cell Function in a Canine Model of Pre-Diabetes.PloS one · 2016Article
- Article
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Authors and funding
15 authors.
Funding
Abstract
objectivesThe canine model has been used extensively to improve the human pancreatic islet isolation technique. At the functional level, dog islets show high similarity to human islets and thus can be a helpful tool for islet research. We describe and compare 2 manual isolation methods, M1 (initial) and M2 (modified), and analyze the variables associated with the outcomes, including islet yield, purity, and glucose-stimulated insulin secretion (GSIS).
methodsMale mongrel dogs were used in the study. M2 (n = 7) included higher collagenase concentration, shorter digestion time, faster shaking speed, colder purification temperature, and higher differential density gradient than M1 (n = 7).
resultsIslet yield was similar between methods (3111.0 ± 309.1 and 3155.8 ± 644.5 islets/g, M1 and M2, respectively; P = 0.951). Pancreas weight and purity together were directly associated with the yield (adjusted R(2) = 0.61; P = 0.002). Purity was considerably improved with M2 (96.7% ± 1.2% vs 75.0% ± 6.3%; P = 0.006). M2 improved GSIS (P = 0.021). Independently, digestion time was inversely associated with GSIS.
conclusionsWe describe an isolation method (M2) to obtain a highly pure yield of dog islets with adequate β-cell glucose responsiveness. The isolation variables associated with the outcomes in our canine model confirm previous reports in other species, including humans.
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