ArticleNeonatology2012
Optimal techniques for mRNA extraction from neonatal salivary supernatant.
Article in Neonatology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 31 citations in OpenAlex.
- A pilot multiplex salivary transcriptomic analysis to understand the sex-specific effects of maternal opioid use in offspring.Scientific reports · 2026Article
- Maternal Opioids Downregulate Adiponectin Receptor Signaling and Alter Growth in Offspring: Pilot Study.medRxiv : the preprint server for health sciences · 2026Article
- Maternal opioids downregulate adiponectin receptor signaling and alter growth in offspring: pilot study.Frontiers in pediatrics · 2026Article
- A Pilot Multiplex Salivary Transcriptomic Analysis to Understand the Sex-specific Effects of Maternal Opioid Use in Offspring.Research square · 2025Article
- The Salivary Transcriptome: A Window into Local and Systemic Gene Expression Patterns.Methods in molecular biology (Clifton, N.J.) · 2025Review
- Assessing the impact of storage conditions on RNA from human saliva and its application to the identification of mRNA biomarkers for asthma.Frontiers in molecular biosciences · 2024Article
- Sex-specific inflammatory and white matter effects of prenatal opioid exposure: a pilot study.Pediatric research · 2023Article
- Salivary C-Reactive Protein and Mean Platelet Volume in the Diagnosis and Follow-Up of Community-Acquired Pneumonia in Infants.Pediatric allergy, immunology, and pulmonology · 2021Observational
- Salivary and Serum Interleukin-10, C-Reactive Protein, Mean Platelet Volume, and CRP/MPV Ratio in the Diagnosis of Late-Onset Neonatal Sepsis in Full-Term Neonates.Journal of immunology research · 2021Observational
- Salivary Interleukin-6 and C-Reactive Protein/Mean Platelet Volume Ratio in the Diagnosis of Late-Onset Neonatal Pneumonia.Journal of immunology research · 2021Observational
- Technical Considerations and Protocol Optimization for Neonatal Salivary Biomarker Discovery and Analysis.Frontiers in pediatrics · 2020Article
- Sex-Dependent Gene Expression in Infants with Neonatal Opioid Withdrawal Syndrome.The Journal of pediatrics · 2019Article
- Somatosensory Modulation of Salivary Gene Expression and Oral Feeding in Preterm Infants: Randomized Controlled Trial.JMIR research protocols · 2017Article
- Salivary FOXP2 expression and oral feeding success in premature infants.Cold Spring Harbor molecular case studies · 2016Article
- FOXP2 gene deletion and infant feeding difficulties: a case report.Cold Spring Harbor molecular case studies · 2016Article
- The Neonatal Salivary Transcriptome.Cold Spring Harbor perspectives in medicine · 2015Review
- Computational gene expression modeling identifies salivary biomarker analysis that predict oral feeding readiness in the newborn.The Journal of pediatrics · 2015Article
- Comparative performance analyses of commercially available products for salivary collection and nucleic acid processing in the newborn.Biotechnic & histochemistry : official publication of the Biological Stain Commission · 2015Article
- Article
- Great expectorations: the potential of salivary 'omic' approaches in neonatal intensive care.Journal of perinatology : official journal of the California Perinatal Association · 2014Review
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundGene expression profiling of the salivary supernatant is emerging as a new and important source of real-time, systemic, biological information. However, existing technologies prevent RNA extraction of small quantities found in neonatal salivary supernatant.
objectiveThe aim of this study was to develop techniques to enhance extraction of cell-free RNA from neonatal salivary supernatant.
methodsTwo saliva samples (10-100 μl) were serially collected from newborns (36-41 weeks' gestation) (n = 13) and stabilized. Total RNA was extracted from salivary supernatant with the use of two modified extraction techniques: Qiagen RNAprotect® Saliva Mini Kit (method 1) and the QIAamp Viral RNA Mini Kit (method 2). Quantitative RT-PCR amplification for GAPDH was performed on extracted salivary samples. Statistical analyses were performed on mean threshold cycle (Ct) levels to compare RNA yield from each protocol. Paired microarray analyses were made between neonatal whole saliva and supernatant (n = 3) to discern gene expression differences between these biolayers.
resultsmRNA was successfully extracted and amplified from all salivary supernatant samples. Extraction with method 2 yielded more RNA than with method 1 (p = 0.008). There was a 7.5% discordance between paired gene expression analyses for whole saliva and supernatant. Genes that were statistically significantly upregulated in supernatant highlighted 16 distinct biological functions not seen in whole saliva. Conversely, only two biological functions were unique to whole saliva.
conclusionNeonatal cell-free salivary supernatant mRNA may be readily extracted and utilized on downstream applications. These technical enhancements allow for further exploration of the diagnostic potential of the neonatal salivary transcriptome.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.