ArticleMolecular and cellular biology2011
The midblastula transition defines the onset of Y RNA-dependent DNA replication in Xenopus laevis.
Article in Molecular and cellular biology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
24 citing papers in PubMed, 37 citations in OpenAlex.
- A guide to the biogenesis and functions of endogenous small non-coding RNAs in animals.Nature reviews. Molecular cell biology · 2025Review
- Noncoding Y RNAs regulate the levels, subcellular distribution and protein interactions of their Ro60 autoantigen partner.Nucleic acids research · 2020Article
- Y RNA: An Overview of Their Role as Potential Biomarkers and Molecular Targets in Human Cancers.Cancers · 2020Review
- Genome wide decrease of DNA replication eye density at the midblastula transition ofCell cycle (Georgetown, Tex.) · 2019Article
- Review
- Ro60 and Y RNAs: structure, functions, and roles in autoimmunity.Critical reviews in biochemistry and molecular biology · 2019Review
- Identification and characterization of stem-bulge RNAs in Drosophila melanogaster.RNA biology · 2019Article
- On the Interplay of the DNA Replication Program and the Intra-S Phase Checkpoint Pathway.Genes · 2019Review
- Bacterial Y RNAs: Gates, Tethers, and tRNA Mimics.Microbiology spectrum · 2018Review
- Innate Immune Response and Off-Target Mis-splicing Are Common Morpholino-Induced Side Effects in Xenopus.Developmental cell · 2018Article
- A NuRD Complex from Xenopus laevis Eggs Is Essential for DNA Replication during Early Embryogenesis.Cell reports · 2018Article
- RNA secondary structure profiling in zebrafish reveals unique regulatory features.BMC genomics · 2018Article
- Nonreplicative functions of the origin recognition complex.Nucleus (Austin, Tex.) · 2018Review
- ORC interacts with THSC/TREX-2 and its subunits promote Nxf1 association with mRNP and mRNA export in Drosophila.Nucleic acids research · 2016Article
- Structural and functional analysis of four non-coding Y RNAs from Chinese hamster cells: identification, molecular dynamics simulations and DNA replication initiation assays.BMC molecular biology · 2016Article
- Functional roles of non-coding Y RNAs.The international journal of biochemistry & cell biology · 2015Review
- Non-coding stem-bulge RNAs are required for cell proliferation and embryonic development in C. elegans.Journal of cell science · 2015Article
- DNA replication origin activation in space and time.Nature reviews. Molecular cell biology · 2015Review
- Short stories on zebrafish long noncoding RNAs.Zebrafish · 2014Review
- Noncoding RNAs in the regulation of DNA replication.Trends in biochemical sciences · 2014Review
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Noncoding Y RNAs are essential for the initiation of chromosomal DNA replication in mammalian cell extracts, but their role in this process during early vertebrate development is unknown. Here, we use antisense morpholino nucleotides (MOs) to investigate Y RNA function in Xenopus laevis and zebrafish embryos. We show that embryos in which Y RNA function is inhibited by MOs develop normally until the midblastula transition (MBT) but then fail to replicate their DNA and die before gastrulation. Consistent with this observation, Y RNA function is not required for DNA replication in Xenopus egg extracts but is required for replication in a post-MBT cell line. Y RNAs do not bind chromatin in karyomeres before MBT, but they associate with interphase nuclei after MBT in an origin recognition complex (ORC)-dependent manner. Y RNA-specific MOs inhibit the association of Y RNAs with ORC, Cdt1, and HMGA1a proteins, suggesting that these molecular associations are essential for Y RNA function in DNA replication. The MBT is thus a transition point between Y RNA-independent and Y RNA-dependent control of vertebrate DNA replication. Our data suggest that in vertebrates Y RNAs function as a developmentally regulated layer of control over the evolutionarily conserved eukaryotic DNA replication machinery.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.