ArticleInternational journal of dermatology2011
The antiproliferative function of violacein-like purple violet pigment (PVP) from an Antarctic Janthinobacterium sp. Ant5-2 in UV-induced 2237 fibrosarcoma.
Article in International journal of dermatology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 27 citations in OpenAlex.
- A bioactive pigment from violet-hued bacteria: a dual approach against prostate cancer and bacterial infections.Antonie van Leeuwenhoek · 2026Article
- In vitro anticancer activity of prodeoxyviolacein produced by3 Biotech · 2025Article
- A comprehensive review on violacein production by microbial fermentation.Biodesign research · 2025Review
- Extracellular vesicles of Janthinobacterium lividum as violacein carriers in melanoma cell treatment.Applied microbiology and biotechnology · 2024Article
- Multi-target drug with potential applications: violacein in the spotlight.World journal of microbiology & biotechnology · 2021Review
- Violacein improves recombinant IgG production by controlling the cell cycle of Chinese hamster ovary cells.Cytotechnology · 2021Article
- Isolation and characterization of violacein from an Antarctic Iodobacter: a non-pathogenic psychrotolerant microorganism.Extremophiles : life under extreme conditions · 2020Article
- The Bacterial Product Violacein Exerts an Immunostimulatory Effect Via TLR8.Scientific reports · 2019Article
- Complete Genome Sequence of a Psychrotolerant Denitrifying Bacterium,Genome announcements · 2017Article
- Draft Genome Sequence of Janthinobacterium sp. Ant5-2-1, Isolated from Proglacial Lake Podprudnoye in the Schirmacher Oasis of East Antarctica.Genome announcements · 2016Article
- Violacein induces p44/42 mitogen-activated protein kinase-mediated solid tumor cell death and inhibits tumor cell migration.Molecular medicine reports · 2015Article
- Violacein induces cell death by triggering mitochondrial membrane hyperpolarization in vitro.BMC microbiology · 2015Article
- UV and cold tolerance of a pigment-producing Antarctic Janthinobacterium sp. Ant5-2.Extremophiles : life under extreme conditions · 2013Article
- The Janthinobacterium sp. HH01 genome encodes a homologue of the V. cholerae CqsA and L. pneumophila LqsA autoinducer synthases.PloS one · 2013Article
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7 authors at 3 institutions in 1 country.
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Abstract
backgroundIn this study, we have investigated the chemotherapeutic potential of a purple violet pigment (PVP), which was isolated from a previously undescribed Antarctic Janthinobacterium sp. (Ant5-2), against murine UV-induced 2237 fibrosarcoma and B16F10 melanoma cells.
methodsThe 2237, B16F10, C50, and NIH3T3 cells were treated with PVP at different doses and for different times, and their proliferation and viability were detected by 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay. Cell cycle arrest induced by PVP in 2237 fibrosarcoma cells was assessed by flow cytometry and expression analysis of cell cycle regulatory proteins were done by Western blot. Apoptosis induced by PVP in 2237 cells was observed by annexin-V/propidium iodide double staining flow cytometry assay and fluorescence microscopy. To further determine the molecular mechanism of apoptosis induced by PVP, the changes in expression of Bcl-2, Bax and cytochrome c were detected by Western blot. The loss of mitochondrial membrane potential in PVP treated 2237 cells was assessed by staining with JC-1 dye following flow cytometry. Caspase-3, Caspase-9 and PARP cleavage were analyzed by Western blot and Caspase-3 and -9 activities were measured by colorimetric assays.
resultsIn vitro treatment of murine 2237 cells with the PVP resulted in decreased cell viability (13-79%) in a time (24-72 h) and dose (0.1-1 μM)-dependent manner. The PVP-induced growth inhibition in 2237 cells was associated with both G0/G1 and G2/M phase arrest accompanied with decrease in the expression of cyclin dependent kinases (Cdks) and simultaneous increase in the expression of cyclin dependent kinase inhibitors (Cdki) - Cip1/p21 and Kip1/p27. Further, we observed a significant increase in the apoptosis of the 2237 fibrosarcoma cells which was associated with an increased expression of pro-apoptotic protein Bax, decreased expression of anti-apoptotic proteins Bcl-2, disruption of mitochondrial membrane potential, cytochrome c release, activation of caspase-3, caspase-9 and poly-ADP-ribose-polymerase (PARP) cleavage.
conclusionsWe describe the anti-cancer mechanism of the PVP for the first time from an Antarctic bacterium and suggest that the PVP could be used as a potent chemotherapeutic agent against nonmelanoma skin cancers.
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