ArticleJournal of plant research2012
Fast loading ester fluorescent Ca2+ and pH indicators into pollen of Pyrus pyrifolia.
Article in Journal of plant research, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed.
- Nontransformation methods for studying signaling pathways and genes involved in Brassica rapa pollen-stigma interactions.Plant physiology · 2024Article
- Loading calcium fluorescent probes into protoplasts to detect calcium in the flesh tissue cells ofHorticulture research · 2020Article
- Calcium Treatment Alleviates Pericarp Browning of 'Nanguo' Pears by Regulating the GABA Shunt After Cold Storage.Frontiers in plant science · 2020Article
- Abscisic Acid Is Required for Root Elongation Associated With CaFrontiers in plant science · 2020Article
- Spermine Regulates Pollen Tube Growth by Modulating CaFrontiers in plant science · 2017Article
- The effect of exogenous calcium on mitochondria, respiratory metabolism enzymes and ion transport in cucumber roots under hypoxia.Scientific reports · 2015Article
- Arabidopsis thaliana calmodulin-like protein CML24 regulates pollen tube growth by modulating the actin cytoskeleton and controlling the cytosolic Ca(2+) concentration.Plant molecular biology · 2014Article
- Calcium imaging perspectives in plants.International journal of molecular sciences · 2014Review
- The novel Solanum tuberosum calcium dependent protein kinase, StCDPK3, is expressed in actively growing organs.Planta · 2012Article
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Loading of Ca(2+)-sensitive fluorescent probes into plant cells is an essential step to measure activities of free Ca(2+) ions in cytoplasm with a fluorescent imaging technique. Fluo-3 is one of the most suitable Ca(2+) indicators for CLSM. We loaded pollen with fluo-3/AM at three different temperatures. Fluo-3/AM was successfully loaded into pollen at both low (4°C) and high (37°C) temperatures. However, high loading temperature was best suited for pollen, because germination rate of pollen and growth of pollen tubes were relatively little impaired and loading time was shortened. Moreover, Ca(2+) distribution increased in the three apertures of pollen after hydration and showed a Ca(2+) gradient, similar to the tip of growing pollen tubes. The same protocol can be used with the AM-forms of other fluorescent dyes for effective labeling. When loading BCECF-AM into pollen at high temperature, the pollen did not show a pH gradient after hydration. Ca(2+) activities and fluxes had the same periodicity as pollen germination, but pH did not show the same phase and mostly lagged behind. However, the clear zone was alkaline when pollen tube growth was slowed or stopped and turned acidic when growth recovered. It is likely that apical pH(i) regulated pollen tube growth.
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Registered trials
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