ArticleCell research2012
Structural basis for the recognition of Asef by adenomatous polyposis coli.
Article in Cell research, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 43 citations in OpenAlex.
- Predicting colorectal cancer risk in FAP patients using patient-specific organoids.Cancer gene therapy · 2025Article
- Potentials of N-Acyl Hydrazones Against Colorectal Cancer: A Mini Review.Anti-cancer agents in medicinal chemistry · 2025Review
- Computer-aided molecular design and optimization of potent inhibitors disrupting APC‒Asef interaction.Acta pharmaceutica Sinica. B · 2024Article
- Article
- Rational design of a sensitivity-enhanced tracer for discovering efficient APC-Asef inhibitors.Nature communications · 2022Article
- From tumor mutational burden to characteristic targets analysis: Identifying the predictive biomarkers and natural product interventions in cancer management.Frontiers in nutrition · 2022Article
- Article
- Double inhibition and activation mechanisms of Ephexin family RhoGEFs.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Conformational Selection Mechanism Provides Structural Insights into the Optimization of APC-Asef Inhibitors.Molecules (Basel, Switzerland) · 2021Article
- Advances and Insights of APC-Asef Inhibitors for Metastatic Colorectal Cancer Therapy.Frontiers in molecular biosciences · 2021Review
- Branched actin networks are assembled on microtubules by adenomatous polyposis coli for targeted membrane protrusion.The Journal of cell biology · 2020Article
- The structural biology of canonical Wnt signalling.Biochemical Society transactions · 2020Review
- Structure-based discovery of small molecule APC-Asef interaction inhibitors: In silico approaches and molecular dynamics simulations.Journal of molecular modeling · 2020Article
- AnFrontiers in genetics · 2020Article
- Genome-wide Analysis Reveals DNA Methylation Alterations in Obesity Associated with High Risk of Colorectal Cancer.Scientific reports · 2019Article
- Truncated Adenomatous Polyposis Coli Mutation Induces Asef-Activated Golgi Fragmentation.Molecular and cellular biology · 2018Article
- Ubiquitylation and degradation of adenomatous polyposis coli by MKRN1 enhances Wnt/β-catenin signaling.Oncogene · 2018Article
- Peptidomimetic inhibitors of APC-Asef interaction block colorectal cancer migration.Nature chemical biology · 2017Article
- Structural mechanism for the arginine sensing and regulation of CASTOR1 in the mTORC1 signaling pathway.Cell discovery · 2016Article
- Structures of the APC-ARM domain in complexes with discrete Amer1/WTX fragments reveal that it uses a consensus mode to recognize its binding partners.Cell discovery · 2015Article
Corrections and comments
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Authors and funding
12 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Adenomatous polyposis coli (APC) regulates cell-cell adhesion and cell migration through activating the APC-stimulated guanine nucleotide-exchange factor (GEF; Asef), which is usually autoinhibited through the binding between its Src homology 3 (SH3) and Dbl homology (DH) domains. The APC-activated Asef stimulates the small GTPase Cdc42, which leads to decreased cell-cell adherence and enhanced cell migration. In colorectal cancers, truncated APC constitutively activates Asef and promotes cancer cell migration and angiogenesis. Here, we report crystal structures of the human APC/Asef complex. We find that the armadillo repeat domain of APC uses a highly conserved surface groove to recognize the APC-binding region (ABR) of Asef, conformation of which changes dramatically upon binding to APC. Key residues on APC and Asef for the complex formation were mutated and their importance was demonstrated by binding and activity assays. Structural superimposition of the APC/Asef complex with autoinhibited Asef suggests that the binding between APC and Asef might create a steric clash between Asef-DH domain and APC, which possibly leads to a conformational change in Asef that stimulates its GEF activity. Our structures thus elucidate the molecular mechanism of Asef recognition by APC, as well as provide a potential target for pharmaceutical intervention against cancers.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.