ArticleThe Journal of general physiology2011
Xenopus laevis oocytes infected with multi-drug-resistant bacteria: implications for electrical recordings.
Article in The Journal of general physiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
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Who cites it
15 citing papers in PubMed, 31 citations in OpenAlex.
- Article
- Effects of temperature on the interaction between amphibian skin bacteria andFrontiers in microbiology · 2023Article
- Aquatic Freshwater Vertebrate Models of Epilepsy Pathology: Past Discoveries and Future Directions for Therapeutic Discovery.International journal of molecular sciences · 2022Review
- Review
- Structural Mechanics of the Alpha-2-Macroglobulin Transformation.Journal of molecular biology · 2022Article
- Advances in the Microbiology of Stenotrophomonas maltophilia.Clinical microbiology reviews · 2021Article
- Patch-Clamp and Perfusion Techniques to Study Ion Channels Expressed inCold Spring Harbor protocols · 2018Article
- Heterologous Protein Expression in theCold Spring Harbor protocols · 2018Article
- The Effects of 4-Hydroxybenzoic Acid Identified from Bamboo (The Malaysian journal of medical sciences : MJMS · 2018Article
- Antimicrobial Resistance inMicrobiology spectrum · 2018Article
- Mutant SOD1 protein increases Nav1.3 channel excitability.Journal of biological physics · 2016Article
- Nuclear microinjection to assess how heterologously expressed proteins impact Ca2+ signals in Xenopus oocytes.Cold Spring Harbor protocols · 2013Article
- Structural insights into neuronal K+ channel-calmodulin complexes.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Carboetomidate inhibits alpha4/beta2 neuronal nicotinic acetylcholine receptors at concentrations affecting animals.Anesthesia and analgesia · 2012Article
- Redefining our menu: communications and reviews.The Journal of general physiology · 2011Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
Abstract
The Xenopus laevis oocyte has been the workhorse for the investigation of ion transport proteins. These large cells have spawned a multitude of novel techniques that are unfathomable in mammalian cells, yet the fickleness of the oocyte has driven many researchers to use other membrane protein expression systems. Here, we show that some colonies of Xenopus laevis are infected with three multi-drug-resistant bacteria: Pseudomonas fluorescens, Pseudomonas putida, and Stenotrophomonas maltophilia. Oocytes extracted from infected frogs quickly (3-4 d) develop multiple black foci on the animal pole, similar to microinjection scars, which render the extracted eggs useless for electrical recordings. Although multi-drug resistant, the bacteria were susceptible to amikacin and ciprofloxacin in growth assays. Supplementing the oocyte storage media with these two antibiotics prevented the appearance of the black foci and afforded oocytes suitable for whole-cell recordings. Given that P. fluorescens associated with X. laevis has become rapidly drug resistant, it is imperative that researchers store the extracted oocytes in the antibiotic cocktail and not treat the animals harboring the multi-drug-resistant bacteria.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.