ArticleBiomaterials2011
Regulation of hepatic stem/progenitor phenotype by microenvironment stiffness in hydrogel models of the human liver stem cell niche.
Article in Biomaterials, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
44 citing papers in PubMed.
- 3D bioprinting technologies and biomaterial-based scaffolds for wound healing: Insights into decellularized tissue-derived bioinks.Regenerative therapy · 2026Review
- Mechanobiology in Stem Cell-Based Bioprinting.Cell proliferation · 2026Review
- Hepatocyte-like cell therapy for end-stage liver disease: From basic science to clinical application.Liver research (Beijing, China) · 2026Review
- Hormonally and chemically defined expansion conditions for organoids of biliary tree Stem Cells.Bioactive materials · 2024Article
- Mechanosensitive hormone signaling promotes mammary progenitor expansion and breast cancer risk.Cell stem cell · 2024Article
- A postnatal network of co-hepato/pancreatic stem/progenitors in the biliary trees of pigs and humans.NPJ Regenerative medicine · 2023Article
- Liver cell therapies: cellular sources and grafting strategies.Frontiers of medicine · 2023Review
- Patch grafting of organoids of stem/progenitors into solid organs can correct genetic-based disease states.Biomaterials · 2022Article
- Design by Nature: Emerging Applications of Native Liver Extracellular Matrix for Cholangiocyte Organoid-Based Regenerative Medicine.Bioengineering (Basel, Switzerland) · 2022Review
- Mouse Models of Liver Parenchyma Injuries and Regeneration.Frontiers in cell and developmental biology · 2022Review
- Patch grafting, strategies for transplantation of organoids into solid organs such as liver.Biomaterials · 2021Article
- Article
- Response differences of HepG2 and Primary Mouse Hepatocytes to morphological changes in electrospun PCL scaffolds.Scientific reports · 2021Article
- Stem Cells and Hydrogels for Liver Tissue Engineering: Synergistic Cure for Liver Regeneration.Stem cell reviews and reports · 2020Review
- Aptamer-Functionalized Natural Protein-Based Polymers as Innovative Biomaterials.Pharmaceutics · 2020Review
- The Overview of Porous, Bioactive Scaffolds as Instructive Biomaterials for Tissue Regeneration and Their Clinical Translation.Pharmaceutics · 2020Review
- Hyaluronan-Based Grafting Strategies for Liver Stem Cell Therapy and Tracking Methods.Stem cells international · 2019Review
- Bioinspired liver scaffold design criteria.Organogenesis · 2018Article
- Cryopreservation protocol for human biliary tree stem/progenitors, hepatic and pancreatic precursors.Scientific reports · 2017Article
- Hyaluronan coating improves liver engraftment of transplanted human biliary tree stem/progenitor cells.Stem cell research & therapy · 2017Article
Corrections and comments
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Authors and funding
9 authors.
Funding
Abstract
Human livers have maturational lineages of cells within liver acini, beginning periportally in stem cell niches, the canals of Hering, and ending in polyploid hepatocytes pericentrally and cholangiocytes in bile ducts. Hepatic stem cells (hHpSCs) in vivo are partnered with mesenchymal precursors to endothelia (angioblasts) and stellate cells, and reside in regulated microenvironments, stem cell niches, containing hyaluronans (HA). The in vivo hHpSC niche is modeled in vitro by growing hHpSC in two-dimensional (2D) cultures on plastic. We investigated effects of 3D microenvironments, mimicking the liver's stem cell niche, on these hHpSCs by embedding them in HA-based hydrogels prepared with Kubota's Medium (KM), a serum-free medium tailored for endodermal stem/progenitors. The KM-HA hydrogels mimicked the niches, matched diffusivity of culture medium, exhibited shear thinning and perfect elasticity under mechanical loading, and had predictable stiffness depending on their chemistry. KM-HA hydrogels, which supported cell attachment, survival and expansion of hHpSC colonies, induced transition of hHpSC colonies towards stable heterogeneous populations of hepatic progenitors depending on KM-HA hydrogel stiffness, as shown by both their gene and protein expression profile. These acquired phenotypes did not show morphological evidence of fibrotic responses. In conclusion, this study shows that the mechanical properties of the microenvironment can regulate differentiation in endodermal stem cell populations.
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Registered trials
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