Evidence map›Paper›PMID 21786403›Full record

ArticleGenesis (New York, N.Y. : 2000)2011

Stella-Cre mice are highly efficient Cre deleters.

Hui Liu, Wei Wang, Su-Kit Chew, Song-Choon Lee, Juan Li, George S Vassiliou, Tony Green, P Andrew Futreal, Allan Bradley, Shujun Zhang and 1 more

Open access · bronzeAbstract read
In one paragraph

Article in Genesis (New York, N.Y. : 2000), 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
0.3field-weighted citation impact, top 40% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 9 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 3 institutions in 2 countries.

Hui LiuCollege of Animal Science and Technology, Huazhong Agriculture University, Wuhan, China.
Wei Wang
Su-Kit Chew
Song-Choon Lee
Juan Li
George S Vassiliou
Tony Green
P Andrew Futreal
Allan Bradley
Shujun Zhang
Pentao Liu
Wellcome Sanger Institute · GBHuazhong Agricultural University · CNUniversity of Cambridge · GB

Funding

Wellcome Trust
6 · The paper itself

Abstract

Cre-loxP recombination is widely used for genetic manipulation of the mouse genome. Here, we report generation and characterization of a new Cre line, Stella-Cre, where Cre expression cassette was targeted to the 3' UTR of the Stella locus. Stella is specifically expressed in preimplantation embryos and in the germline. Cre-loxP recombination efficiency in Stella-Cre mice was investigated at several genomic loci including Rosa26, Jak2, and Npm1. At all the loci examined, we observed 100% Cre-loxP recombination efficiency in the embryos and in the germline. Thus, Stella-Cre mice serve as a very efficient deleter line.

Indexed as

Gene ExpressionRecombination, Genetic3' Untranslated RegionsAnimalsAttachment Sites, MicrobiologicalBacteriophage P1beta-GalactosidaseBlastocystChromosomal Proteins, Non-HistoneEmbryo, MammalianFemaleFlow CytometryGerm CellsIntegrasesJanus Kinase 2Male3' Untranslated Regionsbeta-GalactosidaseChromosomal Proteins, Non-HistoneCre recombinaseDppa3 protein, mouseGt(ROSA)26Sor non-coding RNA, mouseIntegrasesJak2 protein, mouseJanus Kinase 2Npm1 protein, mouseNuclear ProteinsNucleophosminProteinsRepressor ProteinsRNA, Untranslated

Identifiers

PMID21786403
PMCPMC3674523
OpenAlexW2144698098

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.