ArticleNucleic acids research2011
Conformational change of single-stranded RNAs induced by liposome binding.
Article in Nucleic acids research, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 46 citations in OpenAlex.
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- Protocell Effects on RNA Folding, Function, and Evolution.Accounts of chemical research · 2024Article
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- Minimal mRNA uptake and inflammatory response to COVID-19 mRNA vaccine exposure in human placental explants.medRxiv : the preprint server for health sciences · 2023Article
- A Biopharmaceutical Perspective on Higher-Order Structure and Thermal Stability of mRNA Vaccines.Molecular pharmaceutics · 2022Review
- mRNA Lipoplexes with Cationic and Ionizable α-Amino-lipophosphonates: Membrane Fusion, Transfection, mRNA Translation and Conformation.Pharmaceutics · 2022Article
- Lipid membranes modulate the activity of RNA through sequence-dependent interactions.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Ions in the Deep Subsurface of Earth, Mars, and Icy Moons: Their Effects in Combination with Temperature and Pressure on tRNA-Ligand Binding.International journal of molecular sciences · 2021Article
- mRNA vaccine: a potential therapeutic strategy.Molecular cancer · 2021Review
- Lipid vesicles chaperone an encapsulated RNA aptamer.Nature communications · 2018Article
- Liposomes modified with cardiolipin can act as a platform to regulate the potential flux of NADPMetabolic engineering communications · 2016Article
- Properties of Native High-Density Lipoproteins Inspire Synthesis of Actively TargetedAdvanced functional materials · 2016Article
- Liposome membrane can induce self-cleavage of RNA that models the core fragments of hammerhead ribozyme.European biophysics journal : EBJ · 2016Article
- Organelle-mimicking liposome dissociates G-quadruplexes and facilitates transcription.Nucleic acids research · 2014Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The interaction between single-stranded RNAs and liposomes was studied using UV, Fourier Transform Infrared spectroscopy (FTIR) and Circular Dichroism spectroscopy (CD). The effect of the surface characteristics of liposomes, which were composed of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) and modified with cholesterol (Ch) or 1,2-dioleoyl-3-trimethylammonium propane (DOTAP), on the liposome-RNA interaction was investigated. The fluorescence of 6-(p-toluidino)naphthalene-2-sulfonate (TNS) embedded in the liposome surface (ε = 30-40) was decreased in the presence of tRNA, suggesting that single-stranded tRNA could bind onto the liposome. The dehydration of -PO₂⁻-, guanine (G) and cytosine (C) of tRNA molecules in the presence of liposomes suggested both an electrostatic interaction (phosphate backbone of tRNA and trimethylammonium group of POPC, DOTAP) and a hydrophobic interaction (guanine or cytosine of tRNA and aliphatic tail of lipid). The tRNA conformation on the liposome was determined by CD spectroscopy. POPC/Ch (70/30) maintained tRNA conformation without any denaturation, while POPC/DOTAP(70/30) drastically denatured it. The mRNA translation was evaluated in an Escherichia coli cell-free translation system. POPC/Ch(70/30) enhanced expression of green fluorescent protein (GFP) (116%) while POPC/DOTAP(70/30) inhibited (37%), suggesting that the conformation of RNAs was closely related to the translation efficiency. Therefore, single-stranded RNAs could bind to liposomal membranes through electrostatic and hydrophobic attraction, after which conformational changes were induced depending on the liposome characteristics.
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