ArticleJournal of bacteriology2011
SpoIIE is necessary for asymmetric division, sporulation, and expression of sigmaF, sigmaE, and sigmaG but does not control solvent production in Clostridium acetobutylicum ATCC 824.
Article in Journal of bacteriology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 45 citations in OpenAlex.
- Genome-Wide TSS Distribution in Three Related Clostridia with Normalized Capp-Switch Sequencing.Microbiology spectrum · 2022Article
- Sporulation in solventogenic and acetogenic clostridia.Applied microbiology and biotechnology · 2021Review
- Towards continuous industrial bioprocessing with solventogenic and acetogenic clostridia: challenges, progress and perspectives.Journal of industrial microbiology & biotechnology · 2020Review
- Development of Strong Anaerobic Fluorescent Reporters for Clostridium acetobutylicum and Clostridium ljungdahlii Using HaloTag and SNAP-tag Proteins.Applied and environmental microbiology · 2020Article
- SpoVG is Necessary for Sporulation inMicroorganisms · 2020Article
- Pathway dissection, regulation, engineering and application: lessons learned from biobutanol production by solventogenic clostridia.Biotechnology for biofuels · 2020Review
- Transcriptomic and Phenotypic Analysis of aFrontiers in microbiology · 2020Article
- Acidogenesis, solventogenesis, metabolic stress response and life cycle changes in Clostridium beijerinckii NRRL B-598 at the transcriptomic level.Scientific reports · 2019Article
- Functional Expression of the Clostridium ljungdahlii Acetyl-Coenzyme A Synthase in Clostridium acetobutylicum as Demonstrated by a NovelApplied and environmental microbiology · 2018Article
- Engineering Bacillus megaterium for production of functional intracellular materials.Microbial cell factories · 2017Article
- The industrial anaerobe Clostridium acetobutylicum uses polyketides to regulate cellular differentiation.Nature communications · 2017Article
- Diverse mechanisms regulate sporulation sigma factor activity in the Firmicutes.Current opinion in microbiology · 2015Review
- The Clostridium sporulation programs: diversity and preservation of endospore differentiation.Microbiology and molecular biology reviews : MMBR · 2015Review
- Spontaneous large-scale autolysis in Clostridium acetobutylicum contributes to generation of more spores.Frontiers in microbiology · 2015Article
- Improvement of cellulose catabolism in Clostridium cellulolyticum by sporulation abolishment and carbon alleviation.Biotechnology for biofuels · 2014Article
- σK of Clostridium acetobutylicum is the first known sporulation-specific sigma factor with two developmentally separated roles, one early and one late in sporulation.Journal of bacteriology · 2014Article
- Genetic resources for advanced biofuel production described with the Gene Ontology.Frontiers in microbiology · 2014Review
- A genetic system for Clostridium ljungdahlii: a chassis for autotrophic production of biocommodities and a model homoacetogen.Applied and environmental microbiology · 2013Article
- The spore differentiation pathway in the enteric pathogen Clostridium difficile.PLoS genetics · 2013Article
- Novel system for efficient isolation of Clostridium double-crossover allelic exchange mutants enabling markerless chromosomal gene deletions and DNA integration.Applied and environmental microbiology · 2012Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In order to better characterize the initial stages of sporulation past Spo0A activation and the associated solventogenesis in the important industrial and model organism Clostridium acetobutylicum, the spoIIE gene was successfully disrupted and its expression was silenced. By silencing spoIIE, sporulation was blocked prior to asymmetric division, and no mature spores or any distinguishable morphogenetic changes developed. Upon plasmid-based complementation of spoIIE, sporulation was restored, although the number of spores formed was below that of the plasmid control strain. To investigate the impact of silencing spoIIE on the regulation of sporulation, transcript levels of sigF, sigE, and sigG were examined by semiquantitative reverse transcription-PCR, and the corresponding σF, σE, and σG protein levels were determined by Western analysis. Expression of sigF was significantly reduced in the inactivation strain, and this resulted in very low σF protein levels. Expression of sigE was barely detected, and no sigG transcript was detected at all; consequently, no σE or σG proteins were detected. These data suggest an autostimulatory role for σF in C. acetobutylicum, in contrast to the model organism for endospore formation, Bacillus subtilis, and confirm that high-level expression of σF is required for expression of σE and σG. Unlike the σF and σE inactivation strains, the SpoIIE inactivation strain did not exhibit inoculum-dependent solvent formation and produced good levels of solvents from both exponential- and stationary-phase inocula. Thus, we concluded that SpoIIE does not control solvent formation.
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