ArticleApplied and environmental microbiology2011
Detection of Coxiella burnetii in complex matrices by using multiplex quantitative PCR during a major Q fever outbreak in The Netherlands.
Article in Applied and environmental microbiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers, 1 of them a synthesis that pooled it.
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47 citing papers in PubMed, 1 synthesis or guideline pooled it, 94 citations in OpenAlex.
- A systematic knowledge synthesis on the spatial dimensions of Q fever epidemics.Zoonoses and public health · 2019Pooled it
- Direct whole-genome sequencing of Coxiella burnetii from environmental samples using a two-step selective enrichment approach.PLoS neglected tropical diseases · 2026Article
- A Q Fever Outbreak on a Boer Goat Farm in South Korea: Molecular Typing and Serial Monitoring.Animals : an open access journal from MDPI · 2026Article
- Epidemiological investigation and genetic characterization of Coxiella burnetii carried by parasitic ticks in the southeastern coastal region of China.BMC infectious diseases · 2026Article
- Tracking pathogen-related markers with eDNA in natural areas: how environmental factors shape surveillance strategies.Veterinary research · 2026Article
- Molecular detection ofNew microbes and new infections · 2025Article
- Development of Dembo-PCR for cattle in accordance with Japanese livestock quarantine guidelines.The Journal of veterinary medical science · 2025Article
- Foodborne Pathogens Across Different Food Matrices in Sicily (Southern Italy).Pathogens (Basel, Switzerland) · 2024Article
- Molecular Detection and Characterisation ofPathogens (Basel, Switzerland) · 2024Article
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- Development of a Novel Internally Controlled HrpB1 Gene-Based Real-Time qPCR Assay for Detection of Burkholderia pseudomallei.Molecular diagnosis & therapy · 2024Article
- Detection of Tick-Borne Pathogen Coinfections and Coexposures to Foot-and-Mouth Disease, Brucellosis, and Q Fever in Selected Wildlife From Kruger National Park, South Africa, and Etosha National Park, Namibia.Transboundary and emerging diseases · 2024Article
- Two Years afterAnimals : an open access journal from MDPI · 2023Article
- Molecular detection of Coxiella burnetii and Coxiella species in rats and chickens from poultry farms in North West Province, South Africa.Veterinary medicine and science · 2023Article
- A community approach for pathogens and their arthropod vectors (ticks and fleas) in cats of sub-Saharan Africa.Parasites & vectors · 2022Article
- Molecular detection of Coxiella-like endosymbionts and absence of Coxiella burnetii in Amblyomma mixtum from Veracruz, Mexico.Experimental & applied acarology · 2022Article
- Diversity of bacterial pathogens and their antimicrobial resistance profile among commensal rodents in Qatar.Veterinary research communications · 2022Article
- Diverse and atypical manifestations of Q fever in a metropolitan city hospital: Emerging role of next-generation sequencing for laboratory diagnosis of Coxiella burnetii.PLoS neglected tropical diseases · 2022Article
- Surveillance ofVeterinary sciences · 2022Article
- A community approach of pathogens and their arthropod vectors (ticks and fleas) in dogs of African Sub-Sahara.Parasites & vectors · 2021Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Q fever, caused by Coxiella burnetii, is a zoonosis with a worldwide distribution. A large rural area in the southeast of the Netherlands was heavily affected by Q fever between 2007 and 2009. This initiated the development of a robust and internally controlled multiplex quantitative PCR (qPCR) assay for the detection of C. burnetii DNA in veterinary and environmental matrices on suspected Q fever-affected farms. The qPCR detects three C. burnetii targets (icd, com1, and IS1111) and one Bacillus thuringiensis internal control target (cry1b). Bacillus thuringiensis spores were added to samples to control both DNA extraction and PCR amplification. The performance of the qPCR assay was investigated and showed a high efficiency; a limit of detection of 13.0, 10.6, and 10.4 copies per reaction for the targets icd, com1, and IS1111, respectively; and no cross-reactivity with the nontarget organisms tested. Screening for C. burnetii DNA on 29 suspected Q fever-affected farms during the Q fever epidemic in 2008 showed that swabs from dust-accumulating surfaces contained higher levels of C. burnetii DNA than vaginal swabs from goats or sheep. PCR inhibition by coextracted substances was observed in some environmental samples, and 10- or 100-fold dilutions of samples were sufficient to obtain interpretable signals for both the C. burnetii targets and the internal control. The inclusion of an internal control target and three C. burnetii targets in one multiplex qPCR assay showed that complex veterinary and environmental matrices can be screened reliably for the presence of C. burnetii DNA during an outbreak.
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