ArticleApplied and environmental microbiology2011
Comparative 13C metabolic flux analysis of pyruvate dehydrogenase complex-deficient, L-valine-producing Corynebacterium glutamicum.
Article in Applied and environmental microbiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 73 citations in OpenAlex.
- Construction and screening of L-valine high-yieldingFrontiers in microbiology · 2025Article
- System metabolic engineering ofFrontiers in bioengineering and biotechnology · 2023Article
- Transcriptome analysis of L-leucine-producing Corynebacterium glutamicum under the addition of trimethylglycine.Amino acids · 2022Article
- L-valine production in Corynebacterium glutamicum based on systematic metabolic engineering: progress and prospects.Amino acids · 2021Review
- Engineering of microbial cells for L-valine production: challenges and opportunities.Microbial cell factories · 2021Review
- Improvement of l-Valine Production by Atmospheric and Room Temperature Plasma Mutagenesis and High-Throughput Screening inACS omega · 2020Article
- Impact of COJournal of bacteriology · 2019Article
- Metabolic engineering of Corynebacterium glutamicum for improved L-arginine synthesis by enhancing NADPH supply.Journal of industrial microbiology & biotechnology · 2019Article
- Deciphering the Adaptation ofGenes · 2018Article
- Understanding the high L-valine production in Corynebacterium glutamicum VWB-1 using transcriptomics and proteomics.Scientific reports · 2018Article
- A comprehensive evaluation of constraining amino acid biosynthesis in compartmented models for metabolic flux analysis.Metabolic engineering communications · 2017Article
- A new genome-scale metabolic model ofBiotechnology for biofuels · 2017Article
- Review
- Metabolic engineering of an ATP-neutral Embden-Meyerhof-Parnas pathway in Corynebacterium glutamicum: growth restoration by an adaptive point mutation in NADH dehydrogenase.Applied and environmental microbiology · 2015Article
- NADPH-generating systems in bacteria and archaea.Frontiers in microbiology · 2015Review
- OpenFLUX2: (13)C-MFA modeling software package adjusted for the comprehensive analysis of single and parallel labeling experiments.Microbial cell factories · 2014Article
- Engineering Escherichia coli to overproduce aromatic amino acids and derived compounds.Microbial cell factories · 2014Review
- Carbon flux analysis by 13C nuclear magnetic resonance to determine the effect of CO2 on anaerobic succinate production by Corynebacterium glutamicum.Applied and environmental microbiology · 2014Article
- Article
- Engineering of Corynebacterium glutamicum for high-yield L-valine production under oxygen deprivation conditions.Applied and environmental microbiology · 2013Article
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
L-Valine can be formed successfully using C. glutamicum strains missing an active pyruvate dehydrogenase enzyme complex (PDHC). Wild-type C. glutamicum and four PDHC-deficient strains were compared by (13)C metabolic flux analysis, especially focusing on the split ratio between glycolysis and the pentose phosphate pathway (PPP). Compared to the wild type, showing a carbon flux of 69% ± 14% through the PPP, a strong increase in the PPP flux was observed in PDHC-deficient strains with a maximum of 113% ± 22%. The shift in the split ratio can be explained by an increased demand of NADPH for l-valine formation. In accordance, the introduction of the Escherichia coli transhydrogenase PntAB, catalyzing the reversible conversion of NADH to NADPH, into an L-valine-producing C. glutamicum strain caused the PPP flux to decrease to 57% ± 6%, which is below the wild-type split ratio. Hence, transhydrogenase activity offers an alternative perspective for sufficient NADPH supply, which is relevant for most amino acid production systems. Moreover, as demonstrated for L-valine, this bypass leads to a significant increase of product yield due to a concurrent reduction in carbon dioxide formation via the PPP.
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