ArticleApplied and environmental microbiology2011
Persistent, toxin-antitoxin system-independent, tetracycline resistance-encoding plasmid from a dairy Enterococcus faecium isolate.
Article in Applied and environmental microbiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 34 citations in OpenAlex.
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- Comparative Genomic Analysis of Enterococci across Sectors of the One Health Continuum.Microorganisms · 2023Article
- Enterococcal biofilm-A nidus for antibiotic resistance transfer?Journal of applied microbiology · 2022Review
- Molecular Characteristics ofAnimals : an open access journal from MDPI · 2021Article
- Application of Antibiotics/Antimicrobial Agents on Dental Caries.BioMed research international · 2020Review
- Characterization of Four Multidrug Resistance Plasmids Captured from the Sediments of an Urban Coastal Wetland.Frontiers in microbiology · 2017Article
- Whole-genome sequencing reveals transmission of vancomycin-resistant Enterococcus faecium in a healthcare network.Genome medicine · 2016Observational
- Novel plasmid-borne multidrug resistance gene cluster including lsa(E) from a linezolid-resistant Enterococcus faecium isolate of swine origin.Antimicrobial agents and chemotherapy · 2015Article
- Characterization of antibiotic resistance in commensal bacteria from an aquaculture ecosystem.Frontiers in microbiology · 2015Article
- High abundance and diversity of antimicrobial resistance determinants among early vancomycin-resistant Enterococcus faecium in Poland.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2013Article
- Antibiotic administration routes significantly influence the levels of antibiotic resistance in gut microbiota.Antimicrobial agents and chemotherapy · 2013Article
- Antibiotic resistance: how much do we know and where do we go from here?Applied and environmental microbiology · 2011Article
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4 authors at 1 institution in 1 country.
Funding
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Abstract
A tetracycline-resistant (Tet(r)) dairy Enterococcus faecium isolate designated M7M2 was found to carry both tet(M) and tet(L) genes on a 19.6-kb plasmid. After consecutive transfer in the absence of tetracycline, the resistance-encoding plasmid persisted in 99% of the progenies. DNA sequence analysis revealed that the 19.6-kb plasmid contained 28 open reading frames (ORFs), including a tet(M)-tet(L)-mob gene cluster, as well as a 10.6-kb backbone highly homologous (99.9%) to the reported plasmid pRE25, but without an identified toxin-antitoxin (TA) plasmid stabilization system. The derived backbone plasmid without the Tet(r) determinants exhibited a 100% retention rate in the presence of acridine orange, suggesting the presence of a TA-independent plasmid stabilization mechanism, with its impact on the persistence of a broad spectrum of resistance-encoding traits still to be elucidated. The tet(M)-tet(L) gene cluster from M7M2 was functional and transmissible and led to acquired resistance in Enterococcus faecalis OG1RF by electroporation and in Streptococcus mutans UA159 by natural transformation. Southern hybridization showed that both the tet(M) and tet(L) genes were integrated into the chromosome of S. mutans UA159, while the whole plasmid was transferred to and retained in E. faecalis OG1RF. Quantitative real-time reverse transcription-PCR (RT-PCR) indicated tetracycline-induced transcription of both the tet(M) and tet(L) genes of pM7M2. The results indicated that multiple mechanisms might have contributed to the persistence of antibiotic resistance-encoding genes and that the plasmids pM7M2, pIP816, and pRE25 are likely correlated evolutionarily.
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