ArticleApplied and environmental microbiology2011
Defining components of the chromosomal origin of replication of the hyperthermophilic archaeon Pyrococcus furiosus needed for construction of a stable replicating shuttle vector.
Article in Applied and environmental microbiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 25 citations in OpenAlex.
- Integration of ploidy, Orc1/Cdc6 homolog function, and lysine acetylation with phosphate limitation and UV stress responses of Haloferax volcanii.Extremophiles : life under extreme conditions · 2026Article
- Archaeal DNA replication initiation: bridging LUCA's legacy and modern mechanisms.Frontiers in microbiology · 2025Article
- Thermococcus kodakarensis provides a versatile hyperthermophilic archaeal platform for protein expression.Methods in enzymology · 2021Article
- Cas4 Nucleases Define the PAM, Length, and Orientation of DNA Fragments Integrated at CRISPR Loci.Molecular cell · 2018Article
- Role of free DNA ends and protospacer adjacent motifs for CRISPR DNA uptake in Pyrococcus furiosus.Nucleic acids research · 2017Article
- Genome Replication inFrontiers in microbiology · 2017Article
- Random mutagenesis of the hyperthermophilic archaeon Pyrococcus furiosus using in vitro mariner transposition and natural transformation.Scientific reports · 2016Article
- Bipartite recognition of target RNAs activates DNA cleavage by the Type III-B CRISPR-Cas system.Genes & development · 2016Article
- DNA targeting by the type I-G and type I-A CRISPR-Cas systems of Pyrococcus furiosus.Nucleic acids research · 2015Article
- Argonaute of the archaeon Pyrococcus furiosus is a DNA-guided nuclease that targets cognate DNA.Nucleic acids research · 2015Article
- Engineering hydrogen gas production from formate in a hyperthermophile by heterologous production of an 18-subunit membrane-bound complex.The Journal of biological chemistry · 2014Article
- Archaeal chromosome biology.Journal of molecular microbiology and biotechnology · 2014Review
- The RNA- and DNA-targeting CRISPR-Cas immune systems of Pyrococcus furiosus.Biochemical Society transactions · 2013Review
- Genome sequencing of a genetically tractable Pyrococcus furiosus strain reveals a highly dynamic genome.Journal of bacteriology · 2012Article
- Recombinogenic properties of Pyrococcus furiosus strain COM1 enable rapid selection of targeted mutants.Applied and environmental microbiology · 2012Article
- Overview of the genetic tools in the Archaea.Frontiers in microbiology · 2012Article
- Genetics Techniques for Thermococcus kodakarensis.Frontiers in microbiology · 2012Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
We report the construction of a series of replicating shuttle vectors that consist of a low-copy-number cloning vector for Escherichia coli and functional components of the origin of replication (oriC) of the chromosome of the hyperthermophilic archaeon Pyrococcus furiosus. In the process of identifying the minimum replication origin sequence required for autonomous plasmid replication in P. furiosus, we discovered that several features of the origin predicted by bioinformatic analysis and in vitro binding studies were not essential for stable autonomous plasmid replication. A minimum region required to promote plasmid DNA replication was identified, and plasmids based on this sequence readily transformed P. furiosus. The plasmids replicated autonomously and existed in a single copy. In contrast to shuttle vectors based on a plasmid from the closely related hyperthermophile Pyrococcus abyssi for use in P. furiosus, plasmids based on the P. furiosus chromosomal origin were structurally unchanged after transformation and were stable without selection for more than 100 generations.
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