ArticleThe Journal of biological chemistry2011
Epithelial sodium channel regulation by cell surface-associated serum- and glucocorticoid-regulated kinase 1.
Article in The Journal of biological chemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 32 citations in OpenAlex.
- SARS-CoV-2 N protein induces hypokalemia in acute kidney injury mice via ENaC-dependent mechanism.Molecular therapy : the journal of the American Society of Gene Therapy · 2025Article
- Expression profiles of SGK-1 and α-ENaC in minor salivary glands of subjects with xerostomia.Frontiers in dental medicine · 2025Article
- Aldosterone-independent regulation of K + secretion in the distal nephron.Current opinion in nephrology and hypertension · 2024Review
- The Post-Translational Modification Networking in WNK-Centric Hypertension Regulation and Electrolyte Homeostasis.Biomedicines · 2022Review
- Regulation of distal tubule sodium transport: mechanisms and roles in homeostasis and pathophysiology.Pflugers Archiv : European journal of physiology · 2022Review
- Aldosterone-stimulated endothelial epithelial sodium channel (EnNaC) plays a role in cold exposure-induced hypertension in rats.Frontiers in pharmacology · 2022Article
- Article
- Renal Na-handling defect associated with PER1-dependent nondipping hypertension in male mice.American journal of physiology. Renal physiology · 2018Article
- Aldosterone Upregulates Transient Receptor Potential Melastatin 7 (TRPM7).The Journal of biological chemistry · 2016Article
- Serum and Glucocorticoid Regulated Kinase 1 in Sodium Homeostasis.International journal of molecular sciences · 2016Review
- SGK1 regulation by miR-466g in cortical collecting duct cells.American journal of physiology. Renal physiology · 2016Article
- Potential Roles of Amiloride-Sensitive Sodium Channels in Cancer Development.BioMed research international · 2016Review
- Discovery of N-[4-(1H-Pyrazolo[3,4-b]pyrazin-6-yl)-phenyl]-sulfonamides as Highly Active and Selective SGK1 Inhibitors.ACS medicinal chemistry letters · 2015Article
- Activation of mTORC1 in collecting ducts causes hyperkalemia.Journal of the American Society of Nephrology : JASN · 2014Article
- The kidney and hypertension: pathogenesis of salt-sensitive hypertension.Current hypertension reports · 2012Review
- Scaffold protein connector enhancer of kinase suppressor of Ras isoform 3 (CNK3) coordinates assembly of a multiprotein epithelial sodium channel (ENaC)-regulatory complex.The Journal of biological chemistry · 2012Article
- Photodynamic therapy-generated cancer vaccine elicits acute phase and hormonal response in treated mice.Cancer immunology, immunotherapy : CII · 2012Article
- Differential effects of extracellular ATP on chloride transport in cortical collecting duct cells.American journal of physiology. Renal physiology · 2012Article
- Organization of the ENaC-regulatory machinery.Critical reviews in biochemistry and molecular biologyReview
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
Abstract
Serum- and glucocorticoid-regulated kinase 1 (sgk1) participates in diverse biological processes, including cell growth, apoptosis, and sodium homeostasis. In the cortical collecting duct of the kidney, sgk1 regulates sodium transport by stimulating the epithelial sodium channel (ENaC). Control of subcellular localization of sgk1 may be an important mechanism for modulating specificity of sgk1 function; however, which subcellular locations are required for sgk1-regulated ENaC activity in collecting duct cells has yet to be established. Using cell surface biotinylation studies, we detected endogenous sgk1 at the apical cell membrane of aldosterone-stimulated mpkCCD(c14) collecting duct cells. The association of sgk1 with the cell membrane was enhanced when ENaC was co-transfected with sgk1 in kidney cells, suggesting that ENaC brings sgk1 to the cell surface. Furthermore, association of endogenous sgk1 with the apical cell membrane of mpkCCD(c14) cells could be modulated by treatments that increase or decrease ENaC expression at the apical membrane; forskolin increased the association of sgk1 with the apical surface, whereas methyl-β-cyclodextrin decreased the association of sgk1 with the apical surface. Single channel recordings of excised inside-out patches from the apical membrane of aldosterone-stimulated A6 collecting duct cells revealed that the open probability of ENaC was sensitive to the sgk1 inhibitor GSK650394, indicating that endogenous sgk1 is functionally active at the apical cell membrane. We propose that the association of sgk1 with the apical cell membrane, where it interacts with ENaC, is a novel means by which sgk1 specifically enhances ENaC activity in aldosterone-stimulated collecting duct cells.
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