ArticleThe Journal of biological chemistry2011
Molecular determinants of ciliary membrane localization of Trypanosoma cruzi flagellar calcium-binding protein.
Article in The Journal of biological chemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
30 citing papers in PubMed, 42 citations in OpenAlex.
- Next generation genetic screens in kinetoplastids.Nucleic acids research · 2025Review
- BBSome deficiency in Lotmaria passim reveals divergent functions in trypanosomatid parasites.Parasites & vectors · 2025Article
- Protein subcellular relocalization and function of duplicated flagellar calcium binding protein genes in honey bee trypanosomatid parasite.PLoS genetics · 2024Article
- Proximity-Dependent Biotinylation and Identification of Flagellar Proteins in Trypanosoma cruzi.mSphere · 2023Article
- Proximity-dependent biotinylation and identification of flagellar proteins inbioRxiv : the preprint server for biology · 2023Article
- New Vistas in the Biology of the Flagellum-Pathogens (Basel, Switzerland) · 2022Review
- Touching the Surface: Diverse Roles for the Flagellar Membrane in Kinetoplastid Parasites.Microbiology and molecular biology reviews : MMBR · 2020Review
- The BBSome restricts entry of tagged carbonic anhydrase 6 into the cis-flagellum of Chlamydomonas reinhardtii.PloS one · 2020Article
- Treatment of Trypanosoma cruzi with 2-bromopalmitate alters morphology, endocytosis, differentiation and infectivity.BMC cell biology · 2018Article
- Facilitating trypanosome imaging.Experimental parasitology · 2017Article
- Protein transport in growing and steady-state cilia.Traffic (Copenhagen, Denmark) · 2017Review
- Dynamic protein S-palmitoylation mediates parasite life cycle progression and diverse mechanisms of virulence.Critical reviews in biochemistry and molecular biology · 2017Review
- Open Sesame: How Transition Fibers and the Transition Zone Control Ciliary Composition.Cold Spring Harbor perspectives in biology · 2017Review
- The Trypanosoma cruzi Surface, a Nanoscale Patchwork Quilt.Trends in parasitology · 2017Review
- Two flagellar BAR domain proteins in Trypanosoma brucei with stage-specific regulation.Scientific reports · 2016Article
- Flagellar membranes are rich in raft-forming phospholipids.Biology open · 2015Article
- Myristoylated CIL-7 regulates ciliary extracellular vesicle biogenesis.Molecular biology of the cell · 2015Article
- Calcium-dependent membrane association of a flagellar calcium sensor does not require calcium binding.Molecular and biochemical parasitology · 2015Article
- The Flagellar Arginine Kinase in Trypanosoma brucei Is Important for Infection in Tsetse Flies.PloS one · 2015Article
- Acylation in trypanosomatids: an essential process and potential drug target.Trends in parasitology · 2014Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
The flagellar calcium-binding protein (FCaBP) of Trypanosoma cruzi is localized to the flagellar membrane in all life cycle stages of the parasite. Myristoylation and palmitoylation of the N terminus of FCaBP are necessary for flagellar membrane targeting. Not all dually acylated proteins in T. cruzi are flagellar, however. Other determinants of FCaBP therefore likely contribute to flagellar specificity. We generated T. cruzi transfectants expressing the N-terminal 24 or 12 amino acids of FCaBP fused to GFP. Analysis of these mutants revealed that although amino acids 1-12 are sufficient for dual acylation and membrane binding, amino acids 13-24 are required for flagellar specificity and lipid raft association. Mutagenesis of several conserved lysine residues in the latter peptide demonstrated that these residues are essential for flagellar targeting and lipid raft association. Finally, FCaBP was expressed in the protozoan Leishmania amazonensis, which lacks FCaBP. The flagellar localization and membrane association of FCaBP in L. amazonensis suggest that the mechanisms for flagellar targeting, including a specific palmitoyl acyltransferase, are conserved in this organism.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.