ArticleCell2011
The inside-out mechanism of Dicers from budding yeasts.
Article in Cell, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
47 citing papers in PubMed, 71 citations in OpenAlex.
- Pervasive formation of double-stranded RNAs by overlapping sense/antisense transcripts in budding yeast mitosis and meiosis.RNA (New York, N.Y.) · 2025Article
- Fungal small RNA: unveiling the breakthroughs and promising applications.Frontiers in fungal biology · 2025Review
- Identification of an active RNAi pathway inProceedings of the National Academy of Sciences of the United States of America · 2024Article
- Dicer structure and function: conserved and evolving features.EMBO reports · 2023Review
- Identification and Characterization of Development-Related microRNAs in the Red Flour Beetle,International journal of molecular sciences · 2023Article
- A Robust Platform for Unnatural Amino Acid Mutagenesis in E. coli Using the Bacterial Tryptophanyl-tRNA synthetase/tRNA pair.Journal of molecular biology · 2022Article
- A Structural View of miRNA Biogenesis and Function.Non-coding RNA · 2022Review
- Structural basis for Dicer-like function of an engineered RNase III variant and insights into the reaction trajectory of two-MgRNA biology · 2022Article
- Structural and mechanistic insight into stem-loop RNA processing by yeast Pichia stipitis Dicer.Protein science : a publication of the Protein Society · 2021Article
- A ribonuclease III involved in virulence of Mucorales fungi has evolved to cut exclusively single-stranded RNA.Nucleic acids research · 2021Article
- Genetic Insight into the Domain Structure and Functions of Dicer-Type Ribonucleases.International journal of molecular sciences · 2021Review
- A Glimpse of "Dicer Biology" Through the Structural and Functional Perspective.Frontiers in molecular biosciences · 2021Review
- Xrn1p acts at multiple steps in the budding-yeast RNAi pathway to enhance the efficiency of silencing.Nucleic acids research · 2020Article
- Genome-Wide Identification of RNA Silencing-Related Genes and Their Expressional Analysis in Response to Heat Stress in Barley (Biomolecules · 2020Article
- Advances in RNAi-Assisted Strain Engineering inFrontiers in bioengineering and biotechnology · 2020Review
- Dicer 1 ofVirusdisease · 2019Article
- The molecular mechanism of dsRNA processing by a bacterial Dicer.Nucleic acids research · 2019Article
- Structural insights into DNA degradation by human mitochondrial nuclease MGME1.Nucleic acids research · 2018Article
- The siRNA suppressor RTL1 is redox-regulated through glutathionylation of a conserved cysteine in the double-stranded-RNA-binding domain.Nucleic acids research · 2017Article
- Structural and functional studies of a noncanonical Dicer from Entamoeba histolytica.Scientific reports · 2017Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
The Dicer ribonuclease III (RNase III) enzymes process long double-stranded RNA (dsRNA) into small interfering RNAs (siRNAs) that direct RNA interference. Here, we describe the structure and activity of a catalytically active fragment of Kluyveromyces polysporus Dcr1, which represents the noncanonical Dicers found in budding yeasts. The crystal structure revealed a homodimer resembling that of bacterial RNase III but extended by a unique N-terminal domain, and it identified additional catalytic residues conserved throughout eukaryotic RNase III enzymes. Biochemical analyses showed that Dcr1 dimers bind cooperatively along the dsRNA substrate such that the distance between consecutive active sites determines the length of the siRNA products. Thus, unlike canonical Dicers, which successively remove siRNA duplexes from the dsRNA termini, budding-yeast Dicers initiate processing in the interior and work outward. The distinct mechanism of budding-yeast Dicers establishes a paradigm for natural molecular rulers and imparts substrate preferences with ramifications for biological function.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.