ArticleBMC microbiology2011
Electron transport in acetate-grown Methanosarcina acetivorans.
Article in BMC microbiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
34 citing papers in PubMed, 74 citations in OpenAlex.
- Magnetite drives microbial community restructuring and stimulates aceticlastic methanogenesis of type II Methanosarcina in mangrove sediments.Microbiome · 2025Article
- Catalytic Activity of the Archetype from Group 4 of the FTR-like Ferredoxin:Thioredoxin Reductase Family Is Regulated by UniqueBiochemistry · 2024Article
- MmcA is an electron conduit that facilitates both intracellular and extracellular electron transport in Methanosarcina acetivorans.Nature communications · 2024Article
- Model Organisms To Study Methanogenesis, a Uniquely Archaeal Metabolism.Journal of bacteriology · 2023Review
- MmcA is an electron conduit that facilitates both intracellular and extracellular electron transport inbioRxiv : the preprint server for biology · 2023Article
- An Archaea-specificeLife · 2022Article
- DeconstructingProceedings of the National Academy of Sciences of the United States of America · 2022Article
- Comparative genomics reveals electron transfer and syntrophic mechanisms differentiating methanotrophic and methanogenic archaea.PLoS biology · 2022Review
- Article
- Putative Extracellular Electron Transfer in Methanogenic Archaea.Frontiers in microbiology · 2021Review
- Review
- Structure and function of an unusual flavodoxin from the domainProceedings of the National Academy of Sciences of the United States of America · 2019Article
- Energy Conservation and Hydrogenase Function in Methanogenic Archaea, in Particular the GenusMicrobiology and molecular biology reviews : MMBR · 2019Review
- A Membrane-Bound Cytochrome EnablesmBio · 2019Article
- Life on the thermodynamic edge: Respiratory growth of an acetotrophic methanogen.Science advances · 2019Article
- Kinetic modeling of Stickland reactions-coupled methanogenesis for a methanogenic culture.AMB Express · 2019Article
- A biochemical framework for anaerobic oxidation of methane driven by Fe(III)-dependent respiration.Nature communications · 2018Article
- Electron Bifurcation and Confurcation in Methanogenesis and Reverse Methanogenesis.Frontiers in microbiology · 2018Review
- Article
- Methanosarcina acetivorans utilizes a single NADPH-dependent thioredoxin system and contains additional thioredoxin homologues with distinct functions.Microbiology (Reading, England) · 2017Article
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundAcetate is the major source of methane in nature. The majority of investigations have focused on acetotrophic methanogens for which energy-conserving electron transport is dependent on the production and consumption of H₂ as an intermediate, although the great majority of acetotrophs are unable to metabolize H₂. The presence of cytochrome c and a complex (Ma-Rnf) homologous to the Rnf (Rhodobacter nitrogen fixation) complexes distributed in the domain Bacteria distinguishes non-H₂-utilizing Methanosarcina acetivorans from H₂-utilizing species suggesting fundamentally different electron transport pathways. Thus, the membrane-bound electron transport chain of acetate-grown M. acetivorans was investigated to advance a more complete understanding of acetotrophic methanogens.
resultsA component of the CO dehydrogenase/acetyl-CoA synthase (CdhAE) was partially purified and shown to reduce a ferredoxin purified using an assay coupling reduction of the ferredoxin to oxidation of CdhAE. Mass spectrometry analysis of the ferredoxin identified the encoding gene among annotations for nine ferredoxins encoded in the genome. Reduction of purified membranes from acetate-grown cells with ferredoxin lead to reduction of membrane-associated multi-heme cytochrome c that was re-oxidized by the addition of either the heterodisulfide of coenzyme M and coenzyme B (CoM-S-S-CoB) or 2-hydoxyphenazine, the soluble analog of methanophenazine (MP). Reduced 2-hydoxyphenazine was re-oxidized by membranes that was dependent on addition of CoM-S-S-CoB. A genomic analysis of Methanosarcina thermophila, a non-H2-utilizing acetotrophic methanogen, identified genes homologous to cytochrome c and the Ma-Rnf complex of M. acetivorans.
conclusionsThe results support roles for ferredoxin, cytochrome c and MP in the energy-conserving electron transport pathway of non-H₂-utilizing acetotrophic methanogens. This is the first report of involvement of a cytochrome c in acetotrophic methanogenesis. The results suggest that diverse acetotrophic Methanosarcina species have evolved diverse membrane-bound electron transport pathways leading from ferredoxin and culminating with MP donating electrons to the heterodisulfide reductase (HdrDE) for reduction of CoM-S-S-CoB.
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