ArticlePloS one2011
Activation of Akt by the bacterial inositol phosphatase, SopB, is wortmannin insensitive.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 43 citations in OpenAlex.
- Salmonella SopB suppresses post-transcriptionally regulated cytokine release to reduce early tissue inflammation and delay disease progression.Nature communications · 2026Article
- Enzymatic versatility of the salmonella effector SopB enables manipulation of host phosphoinositide signalling.Discover bacteria · 2026Review
- PI5P4K inhibitors: promising opportunities and challenges.The FEBS journal · 2025Review
- Molecular mechanisms of co-infections.EMBO reports · 2025Review
- The battle between bacterial infection and autophagy in aquatic animals.Frontiers in immunology · 2025Review
- Infection biology ofEcoSal Plus · 2024Review
- Article
- Kinase-independent synthesis of 3-phosphorylated phosphoinositides by a phosphotransferase.Nature cell biology · 2022Article
- Label-free relative quantitative proteomics reveals extracellular vesicles as a vehicle forFrontiers in microbiology · 2022Article
- Modification of phosphoinositides by theFrontiers in cellular and infection microbiology · 2022Review
- Article
- Endocytosis and the internalization of pathogenic organisms: focus on phosphoinositides.F1000Research · 2020Review
- Molecular Mechanisms That Define Redox Balance Function in Pathogen-Host Interactions-Is There a Role for Dietary Bioactive Polyphenols?International journal of molecular sciences · 2019Review
- Article
- How Viral and Intracellular Bacterial Pathogens Reprogram the Metabolism of Host Cells to Allow Their Intracellular Replication.Frontiers in cellular and infection microbiology · 2019Review
- Salmonella Typhimurium effector SseI inhibits chemotaxis and increases host cell survival by deamidation of heterotrimeric Gi proteins.PLoS pathogens · 2018Article
- Article
- MYO6 is targeted byProceedings of the National Academy of Sciences of the United States of America · 2017Article
- A second wave of Salmonella T3SS1 activity prolongs the lifespan of infected epithelial cells.PLoS pathogens · 2017Article
- Salmonella Typhimurium disrupts Sirt1/AMPK checkpoint control of mTOR to impair autophagy.PLoS pathogens · 2017Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
Salmonella enterica uses effector proteins translocated by a Type III Secretion System to invade epithelial cells. One of the invasion-associated effectors, SopB, is an inositol phosphatase that mediates sustained activation of the pro-survival kinase Akt in infected cells. Canonical activation of Akt involves membrane translocation and phosphorylation and is dependent on phosphatidyl inositide 3 kinase (PI3K). Here we have investigated these two distinct processes in Salmonella infected HeLa cells. Firstly, we found that SopB-dependent membrane translocation and phosphorylation of Akt are insensitive to the PI3K inhibitor wortmannin. Similarly, depletion of the PI3K regulatory subunits p85α and p85ß by RNAi had no inhibitory effect on SopB-dependent Akt phosphorylation. Nevertheless, SopB-dependent phosphorylation does depend on the Akt kinases, PDK1 and rictor-mTOR. Membrane translocation assays revealed a dependence on SopB for Akt recruitment to Salmonella ruffles and suggest that this is mediated by phosphoinositide (3,4) P(2) rather than phosphoinositide (3,4,5) P(3). Altogether these data demonstrate that Salmonella activates Akt via a wortmannin insensitive mechanism that is likely a class I PI3K-independent process that incorporates some essential elements of the canonical pathway.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.