ArticlePloS one2011
Honey bee PTEN--description, developmental knockdown, and tissue-specific expression of splice-variants correlated with alternative social phenotypes.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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19 citing papers in PubMed, 34 citations in OpenAlex.
- Examining parent-of-origin effects on transcription and RNA methylation in mediating aggressive behavior in honey bees (Apis mellifera).BMC genomics · 2023Article
- Honey bee functional genomics using symbiont-mediated RNAi.Nature protocols · 2023Review
- Extent and complexity of RNA processing in honey bee queen and worker caste development.iScience · 2022Article
- Glucosamine-6-phosphate N-acetyltransferase gene silencing by parental RNA interference in rice leaf folder, Cnaphalocrocis medinalis (Lepidoptera: Pyralidae).Scientific reports · 2022Article
- Ferritin RNA interference inhibits the formation of iron granules in the trophocytes of worker honey bees (Apis mellifera).Scientific reports · 2019Article
- Horizons in the evolution of aging.BMC biology · 2018Review
- Preparation of Single-cohort Colonies and Hormone Treatment of Worker Honeybees to Analyze Physiology Associated with Role and/or Endocrine System.Journal of visualized experiments : JoVE · 2016Article
- DNA base modifications in honey bee and fruit fly genomes suggest an active demethylation machinery with species- and tissue-specific turnover rates.Biochemistry and biophysics reports · 2016Article
- Negligible uptake and transfer of diet-derived pollen microRNAs in adult honey bees.RNA biology · 2016Article
- RNAi and Antiviral Defense in the Honey Bee.Journal of immunology research · 2015Review
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- Nutritional models of foetal programming and nutrigenomic and epigenomic dysregulations of fatty acid metabolism in the liver and heart.Pflugers Archiv : European journal of physiology · 2014Review
- How flies respond to honey bee pheromone: the role of the foraging gene on reproductive response to queen mandibular pheromone.Die Naturwissenschaften · 2014Article
- Insulin-like peptides (AmILP1 and AmILP2) differentially affect female caste development in the honey bee (Apis mellifera L.).The Journal of experimental biology · 2013Article
- Article
- PtdIns(4,5)P2-mediated cell signaling: emerging principles and PTEN as a paradigm for regulatory mechanism.Advances in experimental medicine and biology · 2013Review
- Gustatory perception and fat body energy metabolism are jointly affected by vitellogenin and juvenile hormone in honey bees.PLoS genetics · 2012Article
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
Abstract
backgroundPhosphatase and TENsin (PTEN) homolog is a negative regulator that takes part in IIS (insulin/insulin-like signaling) and Egfr (epidermal growth factor receptor) activation in Drosophila melanogaster. IIS and Egfr signaling events are also involved in the developmental process of queen and worker differentiation in honey bees (Apis mellifera). Here, we characterized the bee PTEN gene homologue for the first time and begin to explore its potential function during bee development and adult life.
resultsHoney bee PTEN is alternatively spliced, resulting in three splice variants. Next, we show that the expression of PTEN can be down-regulated by RNA interference (RNAi) in the larval stage, when female caste fate is determined. Relative to controls, we observed that RNAi efficacy is dependent on the amount of PTEN dsRNA that is delivered to larvae. For larvae fed queen or worker diets containing a high amount of PTEN dsRNA, PTEN knockdown was significant at a whole-body level but lethal. A lower dosage did not result in a significant gene down-regulation. Finally, we compared same-aged adult workers with different behavior: nursing vs. foraging. We show that between nurses and foragers, PTEN isoforms were differentially expressed within brain, ovary and fat body tissues. All isoforms were expressed at higher levels in the brain and ovaries of the foragers. In fat body, isoform B was expressed at higher level in the nurse bees.
conclusionOur results suggest that PTEN plays a central role during growth and development in queen- and worker-destined honey bees. In adult workers, moreover, tissue-specific patterns of PTEN isoform expression are correlated with differences in complex division of labor between same-aged individuals. Therefore, we propose that knowledge on the roles of IIS and Egfr activity in developmental and behavioral control may increase through studies of how PTEN functions can impact bee social phenotypes.
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