ArticlePloS one2011
Analysis of mitochondrial 3D-deformation in cardiomyocytes during active contraction reveals passive structural anisotropy of orthogonal short axes.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Mitochondrial network configuration influences sarcomere and myosin filament structure in striated muscles.Nature communications · 2022Article
- Piezo buffers mechanical stress via modulation of intracellular CaFrontiers in physiology · 2022Article
- Article
- Tuning mitochondrial structure and function to criticality by fluctuation-driven mechanotransduction.Scientific reports · 2020Article
- Mitochondrial mechanosensor in cardiovascular diseases.Vascular biology (Bristol, England) · 2020Review
- Lessons from the Discovery of Mitochondrial Fragmentation (Fission): A Review and Update.Cells · 2019Review
- Mitochondrial Deformation During the Cardiac Mechanical Cycle.Anatomical record (Hoboken, N.J. : 2007) · 2019Article
- Real time mitochondrial dimension measurements.Journal of biological methods · 2019Article
- Bioenergetic Feedback between Heart Cell Contractile Machinery and Mitochondrial 3D Deformations.Biophysical journal · 2018Article
- An integrative appraisal of mechano-electric feedback mechanisms in the heart.Progress in biophysics and molecular biology · 2017Article
- Caveolae in Rabbit Ventricular Myocytes: Distribution and Dynamic Diminution after Cell Isolation.Biophysical journal · 2017Article
- Mechanotransduction and Metabolism in Cardiomyocyte Microdomains.BioMed research international · 2016Review
- Trans-mitochondrial coordination of cristae at regulated membrane junctions.Nature communications · 2015Article
- Cardiac magnetic resonance assessment of interstitial myocardial fibrosis and cardiomyocyte hypertrophy in hypertensive mice treated with spironolactone.Journal of the American Heart Association · 2014Article
- Quantification of cardiomyocyte hypertrophy by cardiac magnetic resonance: implications for early cardiac remodeling.Circulation · 2013Article
- Matters of the heart in bioenergetics: mitochondrial fusion into continuous reticulum is not needed for maximal respiratory activity.Journal of bioenergetics and biomembranes · 2013Review
- Optical imaging of voltage and calcium in cardiac cells & tissues.Circulation research · 2012Review
- What yeast and cardiomyocytes share: ultradian oscillatory redox mechanisms of cellular coherence and survival.Integrative biology : quantitative biosciences from nano to macro · 2012Review
- Selective ion changes during spontaneous mitochondrial transients in intact astrocytes.PloS one · 2011Article
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Authors and funding
6 authors.
Funding
Abstract
The cardiomyocyte cytoskeleton, composed of rigid and elastic elements, maintains the isolated cell in an elongated cylindrical shape with an elliptical cross-section, even during contraction-relaxation cycles. Cardiomyocyte mitochondria are micron-sized, fluid-filled passive spheres distributed throughout the cell in a crystal-like lattice, arranged in pairs sandwiched between the sarcomere contractile machinery, both longitudinally and radially. Their shape represents the extant 3-dimensional (3D) force-balance. We developed a novel method to examine mitochondrial 3D-deformation in response to contraction and relaxation to understand how dynamic forces are balanced inside cardiomyocytes. The variation in transmitted light intensity induced by the periodic lattice of myofilaments alternating with mitochondrial rows can be analyzed by Fourier transformation along a given cardiomyocyte axis to measure mitochondrial deformation along that axis. This technique enables precise detection of changes in dimension of ∼1% in ∼1 µm (long-axis) structures with 8 ms time-resolution. During active contraction (1 Hz stimulation), mitochondria deform along the length- and width-axes of the cell with similar deformation kinetics in both sarcomere and mitochondrial structures. However, significant deformation anisotropy (without hysteresis) was observed between the orthogonal short-axes (i.e., width and depth) of mitochondria during electrical stimulation. The same degree of deformation anisotropy was also found between the myocyte orthogonal short-axes during electrical stimulation. Therefore, the deformation of the mitochondria reflects the overall deformation of the cell, and the apparent stiffness and stress/strain characteristics of the cytoskeleton differ appreciably between the two cardiomyocyte orthogonal short-axes. This method may be applied to obtaining a better understanding of the dynamic force-balance inside cardiomyocytes and of changes in the spatial stiffness characteristics of the cytoskeleton that may accompany aging or pathological conditions.
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