ArticlePloS one2011
The relative importance of topography and RGD ligand density for endothelial cell adhesion.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
40 citing papers in PubMed, 110 citations in OpenAlex.
- UV/Ozone Treatment of Polymer Surfaces to Enhance Cell Adhesion: The Mechanism and Guidelines for Optimization.Langmuir : the ACS journal of surfaces and colloids · 2025Article
- Biomolecule-functionalized dental implant surfaces: Towards augmenting soft tissue integration.Bioactive materials · 2025Review
- Hydrogels with multiple RGD presentations increase cell adhesion and spreading.Acta biomaterialia · 2025Article
- Dynamic hierarchical ligand anisotropy for competing macrophage regulationBioactive materials · 2025Article
- Cellular Signaling at the Nano-Bio Interface: Spotlighting Membrane Curvature.Annual review of physical chemistry · 2025Review
- Research progress in the regulation of endothelial cells and smooth muscle cells using a micro-nanostructure.Biomedical engineering online · 2025Review
- The Effects of Biomimetic Surface Topography on Vascular Cells: Implications for Vascular Conduits.Advanced healthcare materials · 2024Review
- Hydrogels with Independently Controlled Adhesion Ligand Mobility and Viscoelasticity Increase Cell Adhesion and Spreading.bioRxiv : the preprint server for biology · 2024Article
- The influence of physical and spatial substrate characteristics on endothelial cells.Materials today. Bio · 2024Review
- Use of Chitosan from Southern King Crab to Develop Films Functionalized with RGD Peptides for Potential Tissue Engineering Applications.Biomimetics (Basel, Switzerland) · 2023Article
- Role of immediate early genes in the development of salivary gland organoids in polyisocyanopeptide hydrogels.Frontiers in molecular biosciences · 2023Article
- A Comprehensive Review on Collagen Type I Development of Biomaterials for Tissue Engineering: From Biosynthesis to Bioscaffold.Biomedicines · 2022Review
- A critical review of fibrous polyurethane-based vascular tissue engineering scaffolds.Journal of biological engineering · 2022Review
- Chorion-derived extracellular matrix hydrogel and fibronectin surface coatings show similar beneficial effects on endothelialization of expanded polytetrafluorethylene vascular grafts.Materials today. Bio · 2022Article
- Adaptation of cell spreading to varying fibronectin densities and topographies is facilitated by β1 integrins.Frontiers in bioengineering and biotechnology · 2022Article
- Challenges and strategies forBioactive materials · 2021Review
- Silk-Based Materials for Hard Tissue Engineering.Materials (Basel, Switzerland) · 2021Review
- Fabrication of Tapered 3D Microstructure Arrays Using Dual-Exposure Lithography (DEL).Micromachines · 2020Article
- TiOACS biomaterials science & engineering · 2020Article
- Insights into the angiogenic effects of nanomaterials: mechanisms involved and potential applications.Journal of nanobiotechnology · 2020Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The morphology and function of endothelial cells depends on the physical and chemical characteristics of the extracellular environment. Here, we designed silicon surfaces on which topographical features and surface densities of the integrin binding peptide arginine-glycine-aspartic acid (RGD) could be independently controlled. We used these surfaces to investigate the relative importance of the surface chemistry of ligand presentation versus surface topography in endothelial cell adhesion. We compared cell adhesion, spreading and migration on surfaces with nano- to micro-scaled pyramids and average densities of 6×10(2)-6×10(11) RGD/mm(2). We found that fewer cells adhered onto rough than flat surfaces and that the optimal average RGD density for cell adhesion was 6×10(5) RGD/mm(2) on flat surfaces and substrata with nano-scaled roughness. Only on surfaces with micro-scaled pyramids did the topography hinder cell migration and a lower average RGD density was optimal for adhesion. In contrast, cell spreading was greatest on surfaces with 6×10(8) RGD/mm(2) irrespectively of presence of feature and their size. In summary, our data suggest that the size of pyramids predominately control the number of endothelial cells that adhere to the substratum but the average RGD density governs the degree of cell spreading and length of focal adhesion within adherent cells. The data points towards a two-step model of cell adhesion: the initial contact of cells with a substratum may be guided by the topography while the engagement of cell surface receptors is predominately controlled by the surface chemistry.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.