ArticleFrontiers in pharmacology2010
Intra-Laboratory Pre-Validation of a Human Cell Based in vitro Angiogenesis Assay for Testing Angiogenesis Modulators.
Article in Frontiers in pharmacology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
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- Profiling human brain vascular cells using single-cell transcriptomics and organoids.Nature protocols · 2024Review
- Association between [Molecular biology reports · 2021Article
- The cancer angiogenesis co-culture assay: In vitro quantification of the angiogenic potential of tumoroids.PloS one · 2021Article
- A cross-platform approach to characterize and screen potential neurovascular unit toxicants.Reproductive toxicology (Elmsford, N.Y.) · 2020Article
- Secretome of Senescent Adipose-Derived Mesenchymal Stem Cells Negatively Regulates Angiogenesis.International journal of molecular sciences · 2020Article
- A Modular Microscale Granuloma Model for Immune-Microenvironment Signaling StudiesFrontiers in bioengineering and biotechnology · 2020Article
- Reconfigurable open microfluidics for studying the spatiotemporal dynamics of paracrine signalling.Nature biomedical engineering · 2019Article
- Systems Modeling of Developmental Vascular Toxicity.Current opinion in toxicology · 2019Article
- Review
- Maturation of human pluripotent stem cell derived cardiomyocytes is improved in cardiovascular construct.Cytotechnology · 2017Article
- Stable engineered vascular networks from human induced pluripotent stem cell-derived endothelial cells cultured in synthetic hydrogels.Acta biomaterialia · 2016Article
- Microfluidic multiculture assay to analyze biomolecular signaling in angiogenesis.Analytical chemistry · 2015Article
- Novel in vitro cardiovascular constructs composed of vascular-like networks and cardiomyocytes.In vitro cellular & developmental biology. Animal · 2014Article
- Transient proteolytic modification of mesenchymal stromal cells increases lung clearance rate and targeting to injured tissue.Stem cells translational medicine · 2013Article
- Influence of levamisole and other angiogenesis inhibitors on angiogenesis and endothelial cell morphology in vitro.Cancers · 2013Article
- Environmental impact on vascular development predicted by high-throughput screening.Environmental health perspectives · 2011Article
- Hypoxia effects on proangiogenic factors in human umbilical vein endothelial cells: functional role of the peptide somatostatin.Naunyn-Schmiedeberg's archives of pharmacology · 2011Article
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6 authors.
Funding
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Abstract
The developed standardized human cell based in vitro angiogenesis assay was intra-laboratory pre-validated to verify that the method is reliable and relevant for routine testing of modulators of angiogenesis, e.g., pharmaceuticals and industrial chemicals. This assay is based on the earlier published method but it was improved and shown to be more sensitive and rapid than the previous assay. The performance of the assay was assessed by using six reference chemicals, which are widely used pharmaceuticals that inhibit angiogenesis: acetyl salicylic acid, erlotinib, 2-methoxyestradiol, levamisole, thalidomide, and anti-vascular endothelial growth factor. In the intra-laboratory pre-validation, the sensitivity of the assay (upper and lower limits of detection and linearity of response in tubule formation), batch to batch variation in tubule formation between different Master cell bank batches, and precision as well as the reliability of the assay (reproducibility and repeatability) were tested. The pre-set acceptance criteria for the intra-laboratory pre-validation study were met. The relevance of the assay in man was investigated by comparing the effects of reference chemicals and their concentrations to the published human data. The comparison showed a good concordance, which indicates that this human cell based angiogenesis model predicts well the effects in man and has the potential to be used to supplement and/or replace of animal tests.
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