ArticlePLoS pathogens2011
A genome-wide approach to discovery of small RNAs involved in regulation of virulence in Vibrio cholerae.
Article in PLoS pathogens, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
37 citing papers in PubMed, 70 citations in OpenAlex.
- A 3'UTR-derived small RNA modulates the life cycle of the cholera toxin-encoding filamentous phage, CTXϕ.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Modulation ofJournal of bacteriology · 2024Article
- Nucleoid-associated proteins shape the global protein occupancy and transcriptional landscape of a clinical isolate ofmSphere · 2024Article
- Small regulatory RNAs inmicroLife · 2023Review
- Impact of Gene Repression on Biofilm Formation ofFrontiers in microbiology · 2022Article
- RNA-mediated control of cell shape modulates antibiotic resistance in Vibrio cholerae.Nature communications · 2020Article
- Switching fatty acid metabolism by an RNA-controlled feed forward loop.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- The Vc2 Cyclic di-GMP-Dependent Riboswitch of Vibrio cholerae Regulates Expression of an Upstream Putative Small RNA by Controlling RNA Stability.Journal of bacteriology · 2019Article
- Conservation of Small Regulatory RNAs inInternational journal of molecular sciences · 2019Article
- Cyclic di-GMP Positively Regulates DNA Repair in Vibrio cholerae.Journal of bacteriology · 2018Article
- Cross-Regulation between Bacteria and Phages at a Posttranscriptional Level.Microbiology spectrum · 2018Review
- Regulatory RNAs in Virulence and Host-Microbe Interactions.Microbiology spectrum · 2018Review
- Bacterial Small RNAs in Mixed Regulatory Networks.Microbiology spectrum · 2018Review
- Regulatory Hierarchies Controlling Virulence Gene Expression inFrontiers in microbiology · 2018Review
- Prediction of bacterial small RNAs in the RsmA (CsrA) and ToxT pathways: a machine learning approach.BMC genomics · 2017Article
- Article
- Immunity Provided by an Outer Membrane Vesicle Cholera Vaccine Is Due to O-Antigen-Specific Antibodies Inhibiting Bacterial Motility.Infection and immunity · 2017Article
- ToxR Antagonizes H-NS Regulation of Horizontally Acquired Genes to Drive Host Colonization.PLoS pathogens · 2016Article
- Role of Non-coding Regulatory RNA in the Virulence of Human PathogenicFrontiers in microbiology · 2016Review
- Advantages of mixing bioinformatics and visualization approaches for analyzing sRNA-mediated regulatory bacterial networks.Briefings in bioinformatics · 2015Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Small RNAs (sRNAs) are becoming increasingly recognized as important regulators in bacteria. To investigate the contribution of sRNA mediated regulation to virulence in Vibrio cholerae, we performed high throughput sequencing of cDNA generated from sRNA transcripts isolated from a strain ectopically expressing ToxT, the major transcriptional regulator within the virulence gene regulon. We compared this data set with ToxT binding sites determined by pulldown and deep sequencing to identify sRNA promoters directly controlled by ToxT. Analysis of the resulting transcripts with ToxT binding sites in cis revealed two sRNAs within the Vibrio Pathogenicity Island. When deletions of these sRNAs were made and the resulting strains were competed against the parental strain in the infant mouse model of V. cholerae colonization, one, TarB, displayed a variable colonization phenotype dependent on its physiological state at the time of inoculation. We identified a target of TarB as the mRNA for the secreted colonization factor, TcpF. We verified negative regulation of TcpF expression by TarB and, using point mutations that disrupted interaction between TarB and tpcF mRNA, showed that loss of this negative regulation was primarily responsible for the colonization phenotype observed in the TarB deletion mutant.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.