ArticlePLoS pathogens2011
Fluorescence lifetime imaging unravels C. trachomatis metabolism and its crosstalk with the host cell.
Article in PLoS pathogens, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 49 citations in OpenAlex.
- Intrinsic OPTIR imaging and spectroscopy enable label-free monitoring of viral infection in living cells.Analytical and bioanalytical chemistry · 2026Article
- Metabolic changes inmBio · 2024Article
- Fluorescence Microscopy with Deep UV, Near UV, and Visible Excitation forAstrobiology · 2024Article
- Metabolic changes to host cells withbioRxiv : the preprint server for biology · 2023Article
- Dynamic microscopic optical coherence tomography to visualize the morphological and functional micro-anatomy of the airways.Biomedical optics express · 2022Article
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- Impact of First-Line Antimicrobials onFrontiers in microbiology · 2021Article
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- Recent trends in two-photon auto-fluorescence lifetime imaging (2P-FLIM) and its biomedical applications.Biomedical engineering letters · 2019Review
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- Dynamic energy dependency ofThe Journal of biological chemistry · 2018Article
- Mito-xenophagic killing of bacteria is coordinated by a metabolic switch in dendritic cells.Scientific reports · 2017Article
- Metabolic fingerprinting of bacteria by fluorescence lifetime imaging microscopy.Scientific reports · 2017Article
- Article
- Novel Detection Strategy To Rapidly Evaluate the Efficacy of Antichlamydial Agents.Antimicrobial agents and chemotherapy · 2017Article
- Growth ofFrontiers in cellular and infection microbiology · 2017Article
- Targeted Disruption of Chlamydia trachomatis Invasion by in Trans Expression of Dominant Negative Tarp Effectors.Frontiers in cellular and infection microbiology · 2016Article
- AP-1 Transcription Factor Serves as a Molecular Switch between Chlamydia pneumoniae Replication and Persistence.Infection and immunity · 2015Article
- NADPH-generating systems in bacteria and archaea.Frontiers in microbiology · 2015Review
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Chlamydia trachomatis is an obligate intracellular bacterium that alternates between two metabolically different developmental forms. We performed fluorescence lifetime imaging (FLIM) of the metabolic coenzymes, reduced nicotinamide adenine dinucleotides [NAD(P)H], by two-photon microscopy for separate analysis of host and pathogen metabolism during intracellular chlamydial infections. NAD(P)H autofluorescence was detected inside the chlamydial inclusion and showed enhanced signal intensity on the inclusion membrane as demonstrated by the co-localization with the 14-3-3β host cell protein. An increase of the fluorescence lifetime of protein-bound NAD(P)H [τ₂-NAD(P)H] inside the chlamydial inclusion strongly correlated with enhanced metabolic activity of chlamydial reticulate bodies during the mid-phase of infection. Inhibition of host cell metabolism that resulted in aberrant intracellular chlamydial inclusion morphology completely abrogated the τ₂-NAD(P)H increase inside the chlamydial inclusion. τ₂-NAD(P)H also decreased inside chlamydial inclusions when the cells were treated with IFNγ reflecting the reduced metabolism of persistent chlamydiae. Furthermore, a significant increase in τ₂-NAD(P)H and a decrease in the relative amount of free NAD(P)H inside the host cell nucleus indicated cellular starvation during intracellular chlamydial infection. Using FLIM analysis by two-photon microscopy we could visualize for the first time metabolic pathogen-host interactions during intracellular Chlamydia trachomatis infections with high spatial and temporal resolution in living cells. Our findings suggest that intracellular chlamydial metabolism is directly linked to cellular NAD(P)H signaling pathways that are involved in host cell survival and longevity.
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