ArticleProceedings of the National Academy of Sciences of the United States of America2011
Thermodynamics of copper and zinc distribution in the cyanobacterium Synechocystis PCC 6803.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
30 citing papers in PubMed, 59 citations in OpenAlex.
- Integrated analysis of the redoxome and zinc proteome links ribosomal protein oxidation to zinc homeostasis.Redox biology · 2026Article
- A key heavy metal-binding protein orchestrates plant resistance against a geminivirus.Fundamental research · 2026Article
- Important Structural Features of Thiolate-Rich Four-Helix Bundles for Cu(I) Uptake and Removal.Inorganic chemistry · 2023Article
- Emerging Trends of Nanotechnology and Genetic Engineering in Cyanobacteria to Optimize Production for Future Applications.Life (Basel, Switzerland) · 2022Review
- Crystal Structure of the Human Copper Chaperone ATOX1 Bound to Zinc Ion.Biomolecules · 2022Article
- Copper (I) or (II) Replacement of the Structural Zinc Ion in the Prokaryotic Zinc Finger Ros Does Not Result in a Functional Domain.International journal of molecular sciences · 2022Article
- Effects of Novel Photosynthetic Inhibitor [CuLCells · 2022Article
- Metal binding and interdomain thermodynamics of mammalian metallothionein-3: enthalpically favoured CuChemical science · 2022Article
- Copper delivery to an endospore coat protein ofFrontiers in cell and developmental biology · 2022Article
- Copper binding by a unique family of metalloproteins is dependent on kynurenine formation.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Cu Homeostasis in Bacteria: The Ins and Outs.Membranes · 2020Review
- Current knowledge and recent advances in understanding metabolism of the model cyanobacterium Synechocystis sp. PCC 6803.Bioscience reports · 2020Review
- Cytosolic Copper Binding by a Bacterial Storage Protein and Interplay with Copper Efflux.International journal of molecular sciences · 2019Article
- Bacterial copper storage proteins.The Journal of biological chemistry · 2018Review
- Insight into Metal Removal from Peptides that Sequester Copper for Methane Oxidation.Chemistry (Weinheim an der Bergstrasse, Germany) · 2018Article
- Bacterial cytosolic proteins with a high capacity for Cu(I) that protect against copper toxicity.Scientific reports · 2016Article
- Methanobactin and the Link between Copper and Bacterial Methane Oxidation.Microbiology and molecular biology reviews : MMBR · 2016Review
- Article
- HMA6 and HMA8 are two chloroplast Cu+-ATPases with different enzymatic properties.Bioscience reports · 2015Article
- CopM is a novel copper-binding protein involved in copper resistance in Synechocystis sp. PCC 6803.MicrobiologyOpen · 2015Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Copper is supplied to plastocyanin for photosynthesis and cytochrome c oxidase for respiration in the thylakoids of Synechocystis PCC 6803 by the membrane-bound P-type ATPases CtaA and PacS, and the metallochaperone Atx1. We have determined the Cu(I) affinities of all of the soluble proteins and domains in this pathway. The Cu(I) affinities of the trafficking proteins range from 5 × 10(16) to 5 × 10(17) M(-1) at pH 7.0, consistent with values for homologues. Unusually, Atx1 binds Cu(I) significantly tighter than the metal-binding domains (MBDs) of CtaA and PacS (CtaA(N) and PacS(N)), and equilibrium copper exchange constants of approximately 0.2 are obtained for transfer to the MBDs. Dimerization of Atx1 increases the affinity for Cu(I), but the loop 5 His61 residue has little influence. The MBD of the zinc exporter ZiaA (ZiaA(N)) exhibits an almost identical Cu(I) affinity, and Cu(I) exchange with Atx1, as CtaA(N) and PacS(N), and the relative stabilities of the complexes must enable the metallochaperone to distinguish between the MBDs. The binding of potentially competing zinc to the trafficking proteins has been studied. ZiaA(N) has the highest Zn(II) affinity and thermodynamics could be important for zinc removal from the cell. Plastocyanin has a Cu(I) affinity of 2.6 × 10(17) M(-1), 15-fold tighter than that of the Cu(A) site of cytochrome c oxidase, highlighting the need for specific mechanisms to ensure copper delivery to both of these targets. The narrow range of Cu(I) affinities for the cytoplasmic copper proteins in Synechocystis will facilitate relocation when copper is limiting.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.