ArticleMicrobiology (Reading, England)2011
Molecular characterization of the Borrelia burgdorferi in vivo-essential protein PncA.
Article in Microbiology (Reading, England), 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 35 citations in OpenAlex.
- The crystal structure of the Borrelia burgdorferi nicotinamidase BBE22 resolves a long-standing annotation error.FEBS open bio · 2026Article
- Riboflavin Salvage Supports Glycolysis in Borrelia burgdorferi Through Flavin-Dependent NADMolecular microbiology · 2026Article
- TheInfection and immunity · 2026Article
- Comparative genomics of Borrelia lusitaniae.G3 (Bethesda, Md.) · 2026Article
- DNA Binding by BosR Controls RpoS-Dependent and -Independent Gene Expression in Borrelia burgdorferi.Molecular microbiology · 2026Article
- Gene Knockout in Leptospira spp. by CRISPR/Cas9-NHEJ (Non-Homologous End-Joining) and CRISPR-Prime Editing.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Induced protein expression in Leptospira spp. and its application to CRISPR/Cas9 mutant generation.Scientific reports · 2025Article
- Article
- Metabolic modeling predicts unique drug targets inmSystems · 2023Article
- Extensive diversity in RNA termination and regulation revealed by transcriptome mapping for the Lyme pathogen Borrelia burgdorferi.Nature communications · 2023Article
- Extensive diversity in RNA termination and regulation revealed by transcriptome mapping for the Lyme pathogenbioRxiv : the preprint server for biology · 2023Article
- A Novel Breakthrough inFrontiers in microbiology · 2022Article
- BB0562 is a nutritional virulence determinant with lipase activity important for Borrelia burgdorferi infection and survival in fatty acid deficient environments.PLoS pathogens · 2021Article
- Genetic Manipulation ofCurrent issues in molecular biology · 2021Review
- Metallochaperones Are Needed for Mycobacterium tuberculosis and Escherichia coli Nicotinamidase-Pyrazinamidase Activity.Journal of bacteriology · 2020Article
- Primordial origin and diversification of plasmids in Lyme disease agent bacteria.BMC genomics · 2018Article
- Article
- The Riemerella anatipestifer AS87_01735 Gene Encodes Nicotinamidase PncA, an Important Virulence Factor.Applied and environmental microbiology · 2016Article
- Borrelia burgdorferi: Carbon Metabolism and the Tick-Mammal Enzootic Cycle.Microbiology spectrum · 2015Article
- Use of an endogenous plasmid locus for stable in trans complementation in Borrelia burgdorferi.Applied and environmental microbiology · 2015Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
The conversion of nicotinamide to nicotinic acid by nicotinamidase enzymes is a critical step in maintaining NAD(+) homeostasis and contributes to numerous important biological processes in diverse organisms. In Borrelia burgdorferi, the nicotinamidase enzyme, PncA, is required for spirochaete survival throughout the infectious cycle. Mammals lack nicotinamidases and therefore PncA may serve as a therapeutic target for Lyme disease. Contrary to the in vivo importance of PncA, the current annotation for the pncA ORF suggests that the encoded protein may be inactive due to the absence of an N-terminal aspartic acid residue that is a conserved member of the catalytic triad of characterized PncA proteins. Herein, we have used genetic and biochemical strategies to determine the N-terminal sequence of B. burgdorferi PncA. Our data demonstrate that the PncA protein is 24 aa longer than the currently annotated sequence and that pncA translation is initiated from the rare, non-canonical initiation codon AUU. These findings are an important first step in understanding the catalytic function of this in vivo-essential protein.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.