ArticleMolecular cell2011
The β subunit gate loop is required for RNA polymerase modification by RfaH and NusG.
Article in Molecular cell, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 78 papers.
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Who cites it
78 citing papers in PubMed, 109 citations in OpenAlex.
- RfaH licenses RNA polymerase for long-range transcription.bioRxiv : the preprint server for biology · 2026Article
- Selective targeting of a histone-like silencer Sfx to the R6K conjugal transfer operon.Nucleic acids research · 2026Article
- Exploration of the structural and functional diversity in the metamorphic RfaH subfamily.bioRxiv : the preprint server for biology · 2026Article
- Article
- Applying the brakes to transcription: regulation of gene expression by RNA polymerase pausing.Journal of bacteriology · 2025Review
- NusG-Spt5 Transcription Factors: Universal, Dynamic Modulators of Gene Expression.Journal of molecular biology · 2025Review
- Bacteroides expand the functional versatility of a conserved transcription factor and transcribed DNA to program capsule diversity.Nature communications · 2024Article
- Discovering novel inhibitors of RfaH from Klebsiella pneumoniae to combat antimicrobial resistance.Archives of microbiology · 2024Article
- Architecture of the spinach plastid-encoded RNA polymerase.Nature communications · 2024Article
- Sm-like protein Rof inhibits transcription termination factor ρ by binding site obstruction and conformational insulation.Nature communications · 2024Article
- Concerted transformation of a hyper-paused transcription complex and its reinforcing protein.Nature communications · 2024Article
- Sm-like protein Rof inhibits transcription termination factor ρ by binding site obstruction and conformational insulation.bioRxiv : the preprint server for biology · 2023Article
- Article
- RfaH May Oppose Silencing by H-NS and YmoA Proteins during Transcription Elongation.Journal of bacteriology · 2022Article
- Transcription factors modulate RNA polymerase conformational equilibrium.Nature communications · 2022Article
- Metamorphic proteins under a computational microscope: Lessons from a fold-switching RfaH protein.Computational and structural biotechnology journal · 2022Review
- A translational riboswitch coordinates nascent transcription-translation coupling.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Basic mechanisms and kinetics of pause-interspersed transcript elongation.Nucleic acids research · 2021Review
- NusG-dependent RNA polymerase pausing is a frequent function of this universally conserved transcription elongation factor.Critical reviews in biochemistry and molecular biology · 2020Article
- Article
18 more citing papers are in PubMed but not listed here.
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
In all organisms, RNA polymerase (RNAP) relies on accessory factors to complete synthesis of long RNAs. These factors increase RNAP processivity by reducing pausing and termination, but their molecular mechanisms remain incompletely understood. We identify the β gate loop as an RNAP element required for antipausing activity of a bacterial virulence factor RfaH, a member of the universally conserved NusG family. Interactions with the gate loop are necessary for suppression of pausing and termination by RfaH, but are dispensable for RfaH binding to RNAP mediated by the β' clamp helices. We hypothesize that upon binding to the clamp helices and the gate loop RfaH bridges the gap across the DNA channel, stabilizing RNAP contacts with nucleic acid and disfavoring isomerization into a paused state. We show that contacts with the gate loop are also required for antipausing by NusG and propose that most NusG homologs use similar mechanisms to increase RNAP processivity.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.