ArticleVirology journal2011
Simultaneous detection and differentiation of dengue virus serotypes 1-4, Japanese encephalitis virus, and West Nile virus by a combined reverse-transcription loop-mediated isothermal amplification assay.
Article in Virology journal, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed.
- Development and Validation of a Combined RT-LAMP Assay for the Rapid and Sensitive Detection of Dengue Virus in Clinical Samples from Colombia.Diagnostics (Basel, Switzerland) · 2025Article
- Challenges in Direct Detection of Flaviviruses: A Review.Pathogens (Basel, Switzerland) · 2023Review
- Analytical and diagnostic performance characteristics of reverse-transcriptase loop-mediated isothermal amplification assays for dengue virus serotypes 1-4: A scoping review to inform potential use in portable molecular diagnostic devices.PLOS global public health · 2023Article
- Article
- Dengue fever as a reemerging disease in upper Egypt: Diagnosis, vector surveillance and genetic diversity using RT-LAMP assay.PloS one · 2022Article
- Rapid molecular assays for the detection of the four dengue viruses in infected mosquitoes.Gates open research · 2022Article
- Dengue Detection: Advances in Diagnostic Tools from Conventional Technology to Point of Care.Biosensors · 2021Review
- Spatiotemporal analysis of dengue outbreaks in Samanabad town, Lahore metropolitan area, using geospatial techniques.Environmental monitoring and assessment · 2019Article
- Detection of Novel duck reovirus (NDRV) using visual reverse transcription loop-mediated isothermal amplification (RT-LAMP).Scientific reports · 2018Article
- Development and validation of four one-step real-time RT-LAMP assays for specific detection of each dengue virus serotype.PLoS neglected tropical diseases · 2018Article
- Potential Point-of-Care Testing for Dengue Virus in the Field.Journal of clinical microbiology · 2018Article
- Article
- Detection of dengue, west Nile virus, rickettsiosis and leptospirosis by a new real-time PCR strategy.SpringerPlus · 2016Article
- Article
- Development of reverse-transcription loop-mediated isothermal amplification assay for rapid detection and differentiation of dengue virus serotypes 1-4.BMC microbiology · 2015Article
- Development of a pan-serotype reverse transcription loop-mediated isothermal amplification assay for the detection of dengue virus.Diagnostic microbiology and infectious disease · 2015Article
- Article
- Rapid detection and differentiation of dengue virus serotypes by NS1 specific reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay in patients presenting to a tertiary care hospital in Hyderabad, India.Journal of virological methods · 2015Article
- Colorimetric Detection of Dengue by Single Tube Reverse-Transcription-Loop-Mediated Isothermal Amplification.PloS one · 2015Article
- Recombinase Polymerase Amplification Assay for Rapid Diagnostics of Dengue Infection.PloS one · 2015Article
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11 authors.
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No grant is acknowledged in the PubMed record.
Abstract
backgroundRapid identification and differentiation of mosquito-transmitted flaviviruses in acute-phase sera of patients and field-caught vector mosquitoes are important for the prediction and prevention of large-scale epidemics.
resultsWe developed a flexible reverse-transcription loop-mediated isothermal amplification (RT-LAMP) unit for the detection and differentiation of dengue virus serotypes 1-4 (DENV1-4), Japanese encephalitis virus (JEV), and West Nile virus (WNV). The unit efficiently amplified the viral genomes specifically at wide ranges of viral template concentrations, and exhibited similar amplification curves as monitored by a real-time PCR engine. The detection limits of the RT-LAMP unit were 100-fold higher than that of RT-PCR in 5 of the six flaviviruses. The results on specificity indicated that the six viruses in the assay had no cross-reactions with each other. By examining 66 viral strains of DENV1-4 and JEV, the unit identified the viruses with 100% accuracy and did not cross-react with influenza viruses and hantaviruses. By screening a panel of specimens containing sera of 168 patients and 279 pools of field-caught blood sucked mosquitoes, results showed that this unit is high feasible in clinical settings and epidemiologic field, and it obtained results 100% correlated with real-time RT-PCR.
conclusionsThe RT-LAMP unit developed in this study is able to quickly detect and accurately differentiate the six kinds of flaviviruses, which makes it extremely feasible for screening these viruses in acute-phase sera of the patients and in vector mosquitoes without the need of high-precision instruments.
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