ArticleInvestigative ophthalmology & visual science2011
Downregulation of glutamine synthetase via GLAST suppression induces retinal axonal swelling in a rat ex vivo hydrostatic pressure model.
Article in Investigative ophthalmology & visual science, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 35 citations in OpenAlex.
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- Involvement of Microglia in Retinal Ganglion Cell Injury Induced by IOP Elevation in a Rat Ex Vivo Acute Glaucoma Model.Biomedicines · 2025Article
- A Comparative Transcriptome and Proteome Analysis of the Molecular Mechanism Underlying Anterior to Dorsal Eye Rotation in the Celestial-Eye Goldfish (International journal of molecular sciences · 2025Article
- Beneficial and Detrimental Pressure-Related Effects on Inner Neurons in the Adult Porcine In Vitro Retina.Translational vision science & technology · 2023Article
- The neurosteroid allopregnanolone protects retinal neurons by effects on autophagy and GABRs/GABAAutophagy · 2021Article
- Rutin improves glutamate uptake and inhibits glutamate excitotoxicity in rat brain slices.Molecular biology reports · 2021Article
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- A lab-on-a-chip model of glaucoma.Brain and behavior · 2020Article
- Müller Cell Metabolic Signatures: Evolutionary Conservation and Disruption in Disease.Trends in endocrinology and metabolism: TEM · 2020Review
- 5-HT1A Receptor Agonist Promotes Retinal Ganglion Cell Function by Inhibiting OFF-Type Presynaptic Glutamatergic Activity in a Chronic Glaucoma Model.Frontiers in cellular neuroscience · 2019Article
- Additive neuroprotective effects of 24(S)-hydroxycholesterol and allopregnanolone in an ex vivo rat glaucoma model.Scientific reports · 2018Article
- Neurosteroids and oxysterols as potential therapeutic agents for glaucoma and Alzheimer's disease.Neuropsychiatry · 2018Article
- Immunohistochemical Localization of GFAP and Glutamate Regulatory Proteins in Chick Retina and Their Levels of Expressions in Altered Photoperiods.Cellular and molecular neurobiology · 2017Article
- Alpha7 nicotinic acetylcholine receptor agonist promotes retinal ganglion cell function via modulating GABAergic presynaptic activity in a chronic glaucomatous model.Scientific reports · 2017Article
- TSPO activation modulates the effects of high pressure in a rat ex vivo glaucoma model.Neuropharmacology · 2016Article
- 24(S)-Hydroxycholesterol protects the ex vivo rat retina from injury by elevated hydrostatic pressure.Scientific reports · 2016Article
- Effect of adenosine on GLAST expression in the retina of a chronic ocular hypertension rat model.Experimental and therapeutic medicine · 2015Article
- Experimentally Induced Mammalian Models of Glaucoma.BioMed research international · 2015Review
- Neurosteroids are endogenous neuroprotectants in an ex vivo glaucoma model.Investigative ophthalmology & visual science · 2014Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
Abstract
PURPOSE. High levels of glutamate can be toxic to retinal GCs. Thus, effective buffering of extracellular glutamate is important in preserving retinal structure and function. GLAST, a major glutamate transporter in the retina, and glutamine synthetase (GS) regulate extracellular glutamate accumulation and prevent excitotoxicity. This study was an examination of changes in function and expression of GLAST and GS in ex vivo rat retinas exposed to acute increases in ambient pressure. METHODS. Ex vivo rat retinas were exposed to elevated hydrostatic pressure for 24 hours. The expression of GLAST and GS were examined using immunochemistry and real-time PCR analysis. Also examined were the effects of (2S,3S)-3-[3-[4-(trifluoromethyl) benzoylamino] benzyloxy] aspartate (TFB-TBOA), an inhibitor of glutamate transporters, and l-methionine-S-sulfoximine (MSO), an inhibitor of GS. RESULTS. In this acute model, Western blot and real-time RT-PCR analyses revealed that substantially (75 mm Hg), but not moderately (35 mm Hg), elevated pressure depressed GLAST expression, diminished GS activity, and induced axonal swelling between the GC layer and the inner limiting membrane. However, at the moderately elevated pressure (35 mm Hg), administration of either TFB-TBOA or MSO also induced axonal swelling and excitotoxic neuronal damage. MSO did not depress GLAST expression but TFB-TBOA significantly suppressed GS, suggesting that downregulation of GS during pressure loading may result from impaired GLAST expression. CONCLUSIONS. The retina is at risk during acute intraocular pressure elevation due to downregulation of GS activity resulting from depressed GLAST expression.
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